Evaluation of potential genotoxic/cytotoxic effects induced by epoxiconazole and fenpropimorph-based fungicide in bovine lymphocytes in vitro
Evaluation of potential genotoxic/cytotoxic effects induced by epoxiconazole and fenpropimorph-based fungicide in bovine lymphocytes in vitro
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DOI：
10.1080/03601234.2016.1198643
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发表时间：
2016-01-01
影响因子：
2
通讯作者：
Schwarzbacherova, Viera
中科院分区：
文献类型：
--
作者：
Drazovska, Monika;Sivikova, Katarina;Schwarzbacherova, Viera
采用单细胞凝胶电泳和细胞遗传学方法，对培养的牛淋巴细胞进行染色体畸变、姐妹染色单体交换、微核和荧光原位杂交，研究了以环氧康唑/苯丙咪唑为基础的杀菌剂的潜在基因毒性/细胞毒性作用。没有统计学意义上的DNA损伤升高和细胞遗传学终点的增加。3 ~ 15 μ g mL（-1）的杀菌剂作用于牛淋巴细胞24和48h后，细胞有丝分裂和增殖指数明显下降（P < 0.05, P < 0.01, P < 0.001）。同样，在每个供体中，浓度为1.5 ~ 15 μ g mL（-1）的杀菌剂暴露24小时后，细胞分裂阻断增殖指数（CBPI）均受到抑制（P < 0.01, P < 0.001）。
Potential genotoxic/cytotoxic effects of the epoxiconazole/fenpropimorph-based fungicide were investigated using single cell gel electrophoresis and cytogenetic assays: chromosomal aberrations, sister chromatid exchanges, micronuclei and fluorescence in situ hybridization in cultured bovine lymphocytes. No statistically significant elevations of DNA damage and increases in cytogenetic endpoints were seen. However, evident cytotoxic effect presented as a decrease in mitotic and proliferation indices were recorded after exposure of bovine lymphocytes to the fungicide for 24 and 48h at concentrations ranging from 3 to 15 mu g mL(-1) (P < 0.05, P < 0.01, P < 0.001). Similarly, for 24h an inhibition in the cytokinesis block proliferation index (CBPI) was obtained after exposure to the fungicide at concentrations ranging from 1.5 to 15 mu g mL(-1) (P < 0.01, P < 0.001) in each donor.