Cell-cycle-dependent regulation of cell motility and determination of the role of Rac1
Cell-cycle-dependent regulation of cell motility and determination of the role of Rac1
复制标题
DOI：
10.1016/j.yexcr.2004.01.011
复制
发表时间：
2004-05-01
影响因子：
3.7
通讯作者：
Bock, E
中科院分区：
文献类型：
--
作者：
Walmod, PS;Hartmann-Petersen, R;Bock, E
为了研究细胞周期不同阶段的细胞运动性，通过计算机辅助显微镜对来自三种哺乳动物细胞系（L929，BT4 Cn，HeLa）的非同步细胞进行延时记录，用于确定单个细胞的位移。位移用于计算描述细胞运动的三个关键参数：速度、持续时间和扩散速率。所有研究的细胞系均显示G2期的细胞位移低于G1/S期。这是由速度和/或持续时间的减少引起的。运动性降低伴随着形态学变化，反映G2中细胞体积大于G1。此外，L-细胞和HeLa-细胞似乎是在G2期的粘附性较低。转染的L-细胞与组成型活性Rac 1导致的速度和速率的扩散在G2期的水平相比，在G1的控制细胞的一般增加。与此相反，显性负调控基因Rac 1转染后，细胞运动速度减慢，并导致细胞位移，这在G1和G2中是相同的。这些观察结果表明，培养细胞的迁移是以细胞周期依赖的方式进行调节的，Rac 1活性的增强足以延缓G2中细胞位移的减少。(C)2004年爱思唯尔公司All rights reserved.
To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three key parameters describing cell motility: speed, persistence time and rate of diffusion.All investigated cell lines demonstrated a lower cell displacement in the G2 phase than in the G1/S phases. This was caused by a decrease in speed and/or persistence time. The decrease in motility was accompanied by changes in morphology reflecting the larger volume of cells in G2 than in G1. Furthermore, L-cells and HeLa-cells appeared to be less adherent in the G2 phase.Transfection of L-cells with constitutively active Rac1 led to a general increase in the speed and rate of diffusion in G2 to levels comparable to those of control cells in G1. In contrast, transfection with dominant-negative Rac1 reduced cell speed and resulted in cellular displacements, which were identical in G1 and G2.These observations indicate that migration of cultured cells is regulated in a cell-cycle-dependent manner, and that an enhancement of Rac1 activity is sufficient for a delay of the reduced cell displacement otherwise seen in G2. (C) 2004 Elsevier Inc. All rights reserved.