Human diabetic mesenchymal stem cells from peripheral arterial disease patients promote angiogenesis through unique secretome signatures
Human diabetic mesenchymal stem cells from peripheral arterial disease patients promote angiogenesis through unique secretome signatures
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DOI：
10.1016/j.surg.2017.11.018
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发表时间：
2018-04-01
期刊：
影响因子：
3.8
通讯作者：
Brewster, Luke P.
中科院分区：
文献类型：
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作者：
Morris, Andrew D.;Dalal, Sidd;Brewster, Luke P.
背景糖尿病患者发生严重外周动脉疾病并发症的风险增加。间充质干细胞（MSC）可能有助于限制这些并发症。我们的目的是测试糖尿病与健康MSC的血管生成潜力。通过内皮细胞（EC）增殖、迁移和三维发芽测试MSC的血管生成潜力。用5.5、20或40 mM葡萄糖模拟糖尿病条件。采用酶联免疫吸附法对MSC分泌蛋白组进行定量。人主动脉EC对葡萄糖条件最敏感，并用于所有MSC实验。糖尿病骨髓间充质干细胞的三维浸润能力明显高于健康骨髓间充质干细胞（P
Background. Diabetic patients are at increased risk of complications from severe peripheral arterial disease. Mesenchymal stem cells (MSC) may be useful in limiting these complications. Our objective is to test the angiogenic potential of diabetic versus healthy MSCs.Methods. MSCs' angiogenic potential was tested by endothelial cell (EC) proliferation, migration, and 3-dimensional sprouting. Diabetic conditions were simulated with 5.5, 20, or 40 mM glucose. MSC secretome was quantified by enzyme-linked immunosorbent assay.Results. Human aortic ECs were most sensitive to glucose conditions and were used for all MSC experiments. Diabetic MSCs had greater 3-dimensional invasion than healthy MSCs (P