Effective removal of endocrine disrupting-compounds by lignin peroxidase from the white-rot fungus Phanerochaete sordida YK-624
Effective removal of endocrine disrupting-compounds by lignin peroxidase from the white-rot fungus Phanerochaete sordida YK-624
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白腐菌Phanerochaete sordida YK-624木质素过氧化物酶有效去除内分泌干扰物
DOI：
10.1007/s00284-011-0067-2
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发表时间：
2012
影响因子：
2.6
通讯作者：
H.Kawagishi
中科院分区：
文献类型：
--
作者：
J.Wang;N.Majima;H.Hirai;H.Kawagishi
研究了白腐菌SordidaYK-624（YK-LiP 1）木质素过氧化物酶对内分泌干扰物（EDCs）的去除作用。结果表明，YK-LiP 1对5种内分泌干扰物OP、BPA、E1、E2和EE 2的清除效果优于P.处理24 h后，含有YK-LiP 1的反应混合物中的黄孢霉（Pc-LiP）、OP和BPA几乎完全消失。特别是，E2和EE 2的雌激素活性的去除，其显示出比其他EDCs如BPA和OP高得多的雌激素活性，在用YK-LiP 1处理24小时后被去除。OP和BPA的主要代谢产物分别为5，5 ′-双（1，1，3，3-四甲基丁基）-[1，1 ′-联苯]-2，2 ′-二醇和5，5 ′-双-[1-（4-羟基苯基）-1-甲基乙基]-联苯-2，2 ′-二醇。这些结果表明，YK-LiP 1通过这些化合物的氧化聚合在去除EDCs方面是高度有效的。
The removal of endocrine-disrupting compounds (EDCs) by lignin peroxidase from white-rot fungusPhanerochaete sordidaYK-624 (YK-LiP1) was investigated. Five endocrine disruptors,p–t-octylphenol (OP), bisphenol A (BPA), estrone (E1), 17β-estradiol (E2), and ethinylestradiol (EE2) were eliminated by YK-LiP1 more effectively than lignin peroxidase fromP. chrysosporium(Pc-LiP), and OP and BPA were disappeared almost completely in the reaction mixture containing YK-LiP1 after a 24-h treatment. Particularly, the removal of estrogenic activities of E2and EE2, which show much higher estrogenic activities than other EDCs such as BPA and OP, were removed following 24-h treatment with YK-LiP1. Moreover, 5,5′-bis(1,1,3,3-tetramethylbutyl)-[1,1′-biphenyl]-2,2′-diol and 5,5′-bis-[1-(4-hydroxy-phenyl)-1-methyl-ethyl]-biphenyl-2,2′-diol were identified as the main metabolite from OP or BPA, respectively. These results suggest that YK-LiP1 is highly effective in removing of EDCs by the oxidative polymerization of these compounds.