Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo
Influence of Epinastine Hydrochloride, an H1-Receptor Antagonist, on the Function of Mite Allergen-Pulsed Murine Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo
复制标题
H1 受体拮抗剂盐酸依匹斯汀对螨过敏原脉冲的小鼠骨髓源性树突状细胞体外和体内功能的影响
作者：
Ken;K. Asano;K. Kanai;Miyuki Suzuki;H. Suzaki
树突状细胞(DC)在变态反应性免疫反应的发生发展中起着重要作用。然而，H1受体拮抗剂对DC功能的影响尚不清楚。本研究旨在研究日本最著名的组胺H1受体拮抗剂盐酸依匹斯汀(EP)在体内外对粉尘螨(Der F)冲击小鼠骨髓来源的DC的影响。当EP浓度大于25 ng/m L时，可显著抑制Delf刺激的DC产生IL-6、肿瘤坏死因子-α和IL-10。另一方面，EP可增加Der f脉冲DC产生IL-12的能力。鼻腔内滴注Der f冲击的DC可导致鼻腔嗜酸性粒细胞增多，同时鼻腔灌洗液中IL-5水平显著升高。DER冲击和EP处理的DC显著抑制鼻腔嗜酸性粒细胞和降低IL-5。这些结果表明，EP通过调节DC功能抑制Th2免疫应答的发展，从而有利于改善变态反应性疾病的临床状态。
There is established concept that dendritic cells (DCs) play essential roles in the development of allergic immune responses. However, the influence of H1 receptor antagonists on DC functions is not well defined. The aim of the present study was to examine the effect of epinastine hydrochloride (EP), the most notable histamine H1 receptor antagonists in Japan, on Dermatophagoides farinae (Der f)-pulsed mouse bone marrow-derived DCs in vitro and in vivo. EP at more than 25 ng/mL could significantly inhibit the production of IL-6, TNF-α and IL-10 from Der f-pulsed DCs, which was increased by Der f challenge in vitro. On the other hand, EP increased the ability of Der f-pulsed DCs to produce IL-12. Intranasal instillation of Der f-pulsed DCs resulted in nasal eosinophilia associated with a significant increase in IL-5 levels in nasal lavage fluids. Der f-pulsed and EP-treated DCs significantly inhibited nasal eosinophila and reduced IL-5. These results indicate that EP inhibits the development of Th2 immune responses through the modulation of DC functions and results in favorable modification of clinical status of allergic diseases.
氮卓斯汀对豚鼠乳头肌电和机械活动的影响。
影响因子：
5
作者：
Molyvdas,PA;James,FW;Sperelakis,N
通讯作者：
Sperelakis,N
影响因子：
5.5
作者：
Francis, Heather;Glaser, Shannon;Alpini, Gianfranco
通讯作者：
Alpini, Gianfranco