Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes
Sorting nexin 27 interacts with the Cytohesin associated scaffolding protein (CASP) in lymphocytes
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DOI：
10.1016/j.bbrc.2007.05.162
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发表时间：
2007-08-10
影响因子：
3.1
通讯作者：
Pohajdak, Bill
中科院分区：
文献类型：
--
作者：
MacNeil, Adam J.;Mansour, Marc;Pohajdak, Bill
CASP是一种小分子细胞因子诱导蛋白，主要在造血细胞中表达，它与鸟嘌呤核苷酸交换因子Cytoherin/Arno家族成员有关。细胞粘附素激活ARF，这是一组参与囊泡起始的GTP酶。从功能上讲，CASP是一个接头蛋白，包含一个PDZ结构域、一个卷曲的线圈和一个潜在的羧基末端PDZ结合基序，这是我们试图在这里描述的。通过GST下拉和质谱分析，我们鉴定了CASP和分类连接素27(SNX27)之间的新相互作用。在淋巴细胞中，CASP的PDZ结合基序与SNX27的PDZ结构域相互作用。这种蛋白是分选Nexin蛋白家族中的一个独特成员，该家族通常参与细胞内和细胞内的分选机制。内源性SNX27和CASP共同定位于淋巴细胞的早期内体隔室，也存在于转基因研究中。这些结果表明，内体SNX27可能招募CASP来协调细胞内转运和/或信号复合体。(C)2007 Elsevier Inc.保留所有权利。
CASP is a small cytokine-inducible protein, primarily expressed in hematopoetic cells, which associates with members of the Cytohesin/ARNO family of guanine nucleotide-exchange factors. Cytohesins activate ARFs, a group of GTPases involved in vesicular initiation. Functionally, CASP is an adaptor protein containing a PDZ domain, a coiled-coil, and a potential carboxy terminal PDZ-binding motif that we sought to characterize here. Using GST pulldowns and mass spectrometry we identified the novel interaction of CASP and sorting nexin 27 (SNX27). In lymphocytes, CASP's PDZ-binding motif interacts with the PDZ domain of SNX27. This protein is a unique member of the sorting nexin family of proteins, a group generally involved in the endocytic and intracellular sorting machinery. Endogenous SNX27 and CASP co-localize at the early endosomal compartment in lymphocytes and also in transfection studies. These results suggest that endosomal SNX27 may recruit CASP to orchestrate intracellular trafficking and/or signaling complexes. (c) 2007 Elsevier Inc. All rights reserved.