Molecular Characterization of Staphylococcus aureus from Surgical Site Infections in Orthopedic Patients in an Orthopedic Trauma Clinical Medical Center in Shanghai
Molecular Characterization of Staphylococcus aureus from Surgical Site Infections in Orthopedic Patients in an Orthopedic Trauma Clinical Medical Center in Shanghai
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DOI：
10.1089/sur.2014.027
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发表时间：
2015-02-01
影响因子：
2
通讯作者：
Ni, Yu-Xing
中科院分区：
文献类型：
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作者：
Gu, Fei-Fei;Han, Li-Zhong;Ni, Yu-Xing
背景：金黄色葡萄球菌或耐甲氧西林金黄色葡萄球菌（MRSA）是手术部位感染（SSI）的主要原因。本研究的目的是对S.方法：对上海地区骨科患者SSI中分离的82株金黄色葡萄球菌进行分离培养。金黄色葡萄球菌分离株（46株甲氧西林敏感金黄色葡萄球菌[MSSA]和36株MRSA）收集自骨科患者的SSI。根据临床和实验室标准协会（CLSI）指南进行抗菌药物敏感性试验，并检测了多种临床重要的毒素基因。对所有分离株进行序列型、spa型和agr群分析。结果：耐甲氧西林金黄色葡萄球菌对替考拉宁、米诺环素、喹奴普汀-达福普汀、利奈唑胺、莫匹罗星、万古霉素等抗生素敏感。在所有分离株中发现10株pvl阳性分离株（3株MRSA和7株MSSA）。发现8株社区相关MRSA（CA-MRSA）分离株，其中6株属于ST 59-MRSA-IV，但大多数MRSA分离株（20/36，55.6%）属于ST 239-MRSA-III-t030/t037，具有广泛的抗生素耐药性。结论：ST 239-MRSA-III-t030/t037为MSSA的流行性克隆，卫生保健相关MRSA（HA-MRSA）可能是引起MSSA的主要病原菌。骨科患者的金黄色葡萄球菌SSI。
Background: Staphylococcus aureus or methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of surgical site infections (SSIs). The aim of our study was to characterize molecularly S. aureus isolates from SSIs in orthopedic patients in Shanghai, China.Methods: Eighty-two S. aureus isolates (46 methicillin-susceptible Staphylococcus aureus [MSSA] and 36 MRSA) were collected from SSIs in orthopedic patients. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines and a variety of clinically important toxin genes were detected. The sequence type, spa type, and agr group were determined to analyze the genotypes of all the isolates collected. In addition, MRSA isolates were characterized by staphylococcal cassette chromosome mec (SCCmec) type as well.Results: The strains showed susceptibility to antibiotics such as teicoplanin, minocycline, quinupristin-dalfopristin, linezolid, mupirocin, and vancomycin. Ten pvl-positive isolates (three MRSA and seven MSSA) were found among all isolates. Eight community-associated MRSA (CA-MRSA) isolates were found, six of which belonged to ST59-MRSA-IV but most MRSA isolates (20/36, 55.6%) belonged to ST239-MRSA-III-t030/t037 with a wide range of antibiotic resistance. By contrast, MSSA isolates were more diverse in both molecular characterizations and virulence factors, with eight MSSA isolates harboring more than six toxin genes detected.Conclusions: ST239-MRSA-III-t030/t037 was the epidemic clone, and healthcare-associated MRSA (HA-MRSA) strains might be the major pathogen causing S. aureus SSIs in orthopedic patients.