Prevalence of antibodies against orthopoxviruses among residents of Likouala region, Republic of Congo: Evidence for Monkeypox virus exposure
Prevalence of antibodies against orthopoxviruses among residents of Likouala region, Republic of Congo: Evidence for Monkeypox virus exposure
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DOI：
10.4269/ajtmh.2007.77.1150
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发表时间：
2007-12-01
影响因子：
3.3
通讯作者：
Damon, Inger K.
中科院分区：
文献类型：
--
作者：
Lederman, Edith R.;Reynolds, Mary G.;Damon, Inger K.
猴痘病毒是一种人畜共患正痘病毒（OPX），分布于撒哈拉以南非洲西部和中部。我们在刚果共和国利夸拉地区进行了一项横断面血清调查，以评估OPX暴露。使用Nobuto血液过滤条（NBFS）采集全血。使用酶联免疫吸附试验评估IgM和IgG对OPX的滴度。使用卡方检验或Fisher精确检验比较人口统计学和临床特征与血清状态。进行多变量logistic回归分析，以评估与血清状态独立相关的因素。共分析了994份标本; OPX IgM的总体血清阳性率为1.7%。年龄< 25岁降低了OPX暴露的可能性，并且生活在Ngangania村的人具有独立的更高优势（优势比= 33.5，95%置信区间= 7.2-166）。使用NBFS进行血清学调查的血液采集是可行的和实用的。成年人的活动，如狩猎和尸体处理，可能在接触猴痘病毒方面发挥重要作用。
Monkeypox virus is a zoonotic orthopoxvirus (OPX) of west and central sub-Saharan Africa. We conducted a cross-sectional serosurvey in Likouala region, Republic of Congo to assess exposure to OPX. Whole blood was collected using Nobuto blood filter strips (NBFS). Titers of IgM and IgG to OPX were assessed using an enzyme-linked immunosorbent assay. Demographic and clinical characteristics were compared with serostatus using the chi-square test or Fisher's exact test. Multivariate logistic regression was performed to evaluate factors for independent association with serostatus. A total of 994 specimens were analyzed; the overall seroprevalence for OPX IgM was 1.7%. Age < 25 years reduced the likelihood of OPX exposure, and persons living in Ngangania village had independently higher odds (odds ratio = 33.5, 95% confidence interval = 7.2-166). Blood collection for serosurveys using NBFS is feasible and practical. Adult activities such as hunting and carcass preparation may play an important role in exposure to Monkeypox virus.