Direct Electrochemistry of Microsomal Human Flavin-containing Monooxygenases 1 and 3 on Naphthalenethiol Thin Films.
Direct Electrochemistry of Microsomal Human Flavin-containing Monooxygenases 1 and 3 on Naphthalenethiol Thin Films.
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微粒体人含黄素单加氧酶 1 和 3 在萘硫醇薄膜上的直接电化学。
DOI：
10.1149/2.003312eel
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发表时间：
2013
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Ikegami;M.;Mie;Y.;Hirano;Y. and Komatsu;Y.
含黄素单加氧酶（FMOs）在外源化合物的解毒中起着重要作用，类似于细胞色素P450。我们在这里展示了固定在萘硫酚（Np-S）涂层电极上的人FMO 1或FMO 3微粒体的直接电化学。两种hFMO微粒体都在Np-S薄膜上成功活化，并通过直接电子转移氧化甲基对甲苯基硫醚。米氏常数与溶液相实验中报道的米氏常数相当。该系统不需要NADPH和酶纯化步骤，为构建具有成本效益的传感器提供了潜在的方法。
Flavin-containing monooxygenases (FMOs) play an essential role in the detoxification of foreign compounds, similar to cytochrome P450. We present here the direct electrochemistry of human FMO1 or FMO3 microsomes immobilized on a naphthalenethiol (Np-S)-coated electrode. Both hFMO microsomes were successfully activated on an Np-S thin film and oxidized methyl p-tolyl sulfide through direct electron transfer. The Michaelis constants were comparable to those reported in solution phase experiments. This system, which requires no NADPH and no enzyme purification step, offers a potential method for constructing cost-effective sensors.