Novel Method of Measuring Cantilever Deflection during an AFM Force Measurement.
Novel Method of Measuring Cantilever Deflection during an AFM Force Measurement.
复制标题
在 AFM 力测量过程中测量悬臂变形的新方法。
DOI：
10.1021/la960450y
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Pungor,A
中科院分区：
文献类型：
--
作者：
Hlady,V;Pierce,M;Pungor,A
使用反射干涉对比显微镜（RICM）和原子力显微镜（AFM）的组合，在使用链霉亲和素-生物素识别对的力测量期间监测悬臂-表面分离距离。RICM表明，在AFM检测系统测量粘合剂结合的最终破裂之前，悬臂与表面失去接触。这一发现表明，生物素的物理吸附的白蛋白和随后的结合链霉亲和素的固定化可能已经创建了一个交联的蛋白质网络，其凝聚力通过AFM悬臂梁与固定化的生物素配体进行测试。
A combination of a reflection interference contrast microscope (RICM) and the atomic force microscope (AFM) was used to monitor the cantilever−surface separation distance during force measurements using the streptavidin−biotin recognition pairs. The RICM showed that the cantilever loses contact with the surface before the final rupture of the adhesive bonds is measured by the AFM detection system. This finding suggests that the immobilization of biotin by physisorbed albumin and subsequent binding of streptavidin might have created a cross-linked protein network whose cohesion is tested by the AFM cantilever with the immobilized biotin ligands.