Use of 6-p-toluidino-2-naphthalenesulfonic acid to quantitate lipids after thin-layer chromatography
Use of 6-p-toluidino-2-naphthalenesulfonic acid to quantitate lipids after thin-layer chromatography
复制标题
使用 6-p-甲苯胺基-2-萘磺酸在薄层色谱法后定量脂质
DOI：
10.1016/s0021-9673(00)97644-1
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发表时间：
1982
影响因子：
4.1
通讯作者：
P. Horowitz
中科院分区：
文献类型：
--
作者：
Marjorie A. Jones;R. W. Keenan;P. Horowitz
在薄层色谱（TLC）上检测脂类的方法中，最有用和最普遍适用的方法之一是使用在疏水环境中检测的化合物。一些报道描述了使用s -苯胺萘磺酸盐（ANS）获得的结果。在本报告中。我们提出的数据表明，6-对甲苯胺-2-萘磺酸（TNS）在薄层色谱上检测脂质时比ANS更敏感，因为它的安息量子产率。我们已经证明，使用TNS可以检测和定量非常少量的脂质化合物，如胆固醇。此外。介绍了使用TNS程序等检测方法的其他几个优点。
One of the most useful and generally applicable of a11 of the methods for the detection of lipids on thin-layer chromatography (TLC) is the use of compounds that Buoresce in hydrophobic environments_ Several reports’” have described the results obtained using S-anilinonaphthaIene-I-sulfonate (ANS). In this report. we present ‘data which demonstrate that 6-p-toluidino-2-naphthalenesulfonic acid (TNS) is considerably more sensitive in the detection of lipids on thin-layer chromatograms than ANS because of its rester quantum yield. We have shown that very small amounts of lipid compounds such as cholesterol can bc detected and quantitated using TNS. In addition. several other advantages in using a detection method such as the TNS procedure are presented.