Analytical Enantioseparation of β-Substituted-2-Phenylpropionic Acids by High-Performance Liquid Chromatography with Hydroxypropyl-β-Cyclodextrin as Chiral Mobile Phase Additive.
Analytical Enantioseparation of β-Substituted-2-Phenylpropionic Acids by High-Performance Liquid Chromatography with Hydroxypropyl-β-Cyclodextrin as Chiral Mobile Phase Additive.
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DOI：
10.1093/chromsci/bmv192
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发表时间：
2016-04
影响因子：
1.3
通讯作者：
Shengqiang Tong;Hu Zhang;Jizhong Yan
中科院分区：
文献类型：
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作者：
Shengqiang Tong;Hu Zhang;Jizhong Yan
以羟丙基-β-环糊精(HP-β-CD)为手性流动相添加剂，建立了5种β-取代-2-苯基丙酸对映体的高效液相色谱分析方法，并对其保留机理进行了研究。考察了有机改性剂、不同ODSC18柱、HP-β-CD浓度等因素对测定结果的影响。流动相为甲醇或乙腈-0.5%醋酸三乙胺缓冲液(pH 3.0)+25mmolHP-β-CD(-1)，所有外消旋体均可达到基线分离。在保留机理方面，研究发现，在恒定pH值和柱温条件下，流动相中HP-β-CD的浓度与保留因子呈负相关。
Analytical enantioseparation of five β-substituted-2-phenylpropionic acids by high-performance liquid chromatography with hydroxypropyl-β-cyclodextrin (HP-β-CD) as chiral mobile phase additive was established in this paper, and chromatographic retention mechanism was studied. The effects of various factors such as the organic modifier, different ODS C18 columns and concentration of HP-β-CD were investigated. The chiral mobile phase was composed of methanol or acetonitrile and 0.5% triethylamine acetate buffer at pH 3.0 added with 25 mmol L(-1) of HP-β-CD, and baseline separations could be reached for all racemates. As for chromatographic retention mechanism, it was found that there was a negative correlation between the concentration of HP-β-CD in mobile phase and the retention factor under constant pH value and column temperature.