BUBBLE-INDUCED COLOR DOPPLER FEEDBACK CORRELATES WITH HISTOTRIPSY-INDUCED DESTRUCTION OF STRUCTURAL COMPONENTS IN LIVER TISSUE
BUBBLE-INDUCED COLOR DOPPLER FEEDBACK CORRELATES WITH HISTOTRIPSY-INDUCED DESTRUCTION OF STRUCTURAL COMPONENTS IN LIVER TISSUE
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DOI：
10.1016/j.ultrasmedbio.2017.11.012
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发表时间：
2018-03-01
影响因子：
2.9
通讯作者：
Xu, Zhen
中科院分区：
文献类型：
--
作者：
Macoskey, Jonathan J.;Zhang, Xi;Xu, Zhen
气泡诱导彩色多普勒（BCD）是一种组织碎石治疗监测技术，其使用多普勒超声跟踪在组织碎石气泡云破裂后持续存在的残余空化核的运动。在本研究中，BCD用于在组织破坏组织治疗期间监测组织分级，BCD信号与组织学鉴定的结构和非结构成分的破坏相关，以进一步了解BCD如何监测治疗程度。使用500 kHz、112元件的相控组织摧毁术阵列，通过扫描超过219个位置，每个位置30-1000个脉冲，在离体牛肝组织内产生约6- x 6- x 7-mm的损伤。在所有治疗过程中，使用128元件L7-4成像探头采集BCD信号。BCD信号，然后定量分析使用的时间到峰值反弹速度（t（prv））度量。使用Pearson相关系数，将t（prv）与使用0.001的显著性水平的由不同数量的脉冲产生的病变的组织学分析进行比较。本研究中的组织学分析包括活细胞计数、网织蛋白染色的III型胶原面积和三色染色的I型胶原面积。发现t（prv）度量与网状蛋白染色的III型胶原面积的变化具有统计学显著相关性，Pearson相关系数为-0.94（p < 0.001），表明BCD的变化更可能是由于组织结构组分的破坏。(C)2018年世界医学和生物学超声联合会。All rights reserved.
Bubble-induced color Doppler (BCD) is a histotripsy-therapy monitoring technique that uses Doppler ultrasound to track the motion of residual cavitation nuclei that persist after the collapse of the histotripsy bubble cloud. In this study, BCD is used to monitor tissue fractionation during histotripsy tissue therapy, and the BCD signal is correlated with the destruction of structural and non-structural components identified histologically to further understand how BCD monitors the extent of treatment. A 500-kHz, 112-element phased histotripsy array is used to generate approximately 6- x 6- x 7-mm lesions within ex vivo bovine liver tissue by scanning more than 219 locations with 30-1000 pulses per location. A 128-element L7-4 imaging probe is used to acquire BCD signals during all treatments. The BCD signal is then quantitatively analyzed using the time-to-peak rebound velocity (t(prv)) metric. Using the Pearson correlation coefficient, the t(prv) is compared with histologic analytics of lesions generated by various numbers of pulses using a significance level of 0.001. Histologic analytics in this study include viable cell count, reticulin-stained type III collagen area and trichrome-stained type I collagen area. It is found that the t(prv) metric has a statistically significant correlation with the change in reticulin-stained type III collagen area with a Pearson correlation coefficient of -0.94 (p < 0.001), indicating that changes in BCD are more likely because of destruction of the structural components of tissue. (C) 2018 World Federation for Ultrasound in Medicine & Biology. All rights reserved.