A 43‐base‐pair complementary DNA sequence homology and triplet repeat motif among putative polymeric immunoglobulin receptor messenger RNAs in regenerating rat liver
A 43‐base‐pair complementary DNA sequence homology and triplet repeat motif among putative polymeric immunoglobulin receptor messenger RNAs in regenerating rat liver
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再生大鼠肝脏中假定的聚合免疫球蛋白受体信使RNA之间的43碱基对互补DNA序列同源性和三联体重复基序
DOI：
10.1002/hep.1840180138
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发表时间：
1993
期刊：
影响因子：
13.5
通讯作者：
H. Leffert
中科院分区：
文献类型：
--
作者：
K. S. Koch;R. Fletcher;M. Grond;H. Leffert
我们实验室对控制肝细胞增殖很感兴趣。最近，我们描述了部分肝切除术后肝再生过程中的细胞周期蛋白信使RNA（mRNA）和p32/p34 CDK 2 'cd' 2组蛋白H1-激酶级联反应（1）。该过程还诱导胎儿和新生儿基因以及大量原癌基因的模式化再表达（2）。在从24小时再生肝脏中筛选大鼠poly（A）+互补DNA（cDNA）文库时（由X. P. Lu在本实验室构建）以稍微降低的严格性（1），用编码全长细胞周期蛋白D1 mRNA的1，245-bp人cDNA探针（31）我们从Stratagene Lambda Uni-ZapXR质粒表达载体（Stratagene，拉霍亚，CA）（见图1和“证明1中添加的注释”。到目前为止，在部分DNA测序后，没有一个插入片段显示出与人细胞周期蛋白D1 mRNA的显著同源性（3）（通过Harr图或通过多重比对构建和分析方法[MACAW程序[41]），尽管在四个cDNA中观察到几个富含d[GAl]的相似区域（图2）。令人惊讶的是，NCBI数据库BLAST搜索扫描了81，327个DNA序列，发现每个大鼠插入片段包含43 bp同源性的完美匹配（阴影框，图2）至BMPF 1的3’-UTR的核苷酸3227-3269，BMPF 1是最近用于确定3，大鼠多聚IgA受体mRNA的269-bp cDNA序列（5）（PolyIgR;图1）。MACAW序列比对搜索未能揭示人类细胞周期蛋白D1和这种受体之间的显着相似性。然而，其中一个插入片段（克隆1）分别沿位置1-68和3202-3269之间的68-bp延伸沿着与DF 1完全匹配，如图1所示（P（N）15 × lop 2 ′）。尚未报道大鼠多聚免疫球蛋白受体3269位以外的cDNA序列（图1中相对序列位置79）。因此，大鼠伊加受体mRNA的未翻译的3 '-UTR的结构是未知的。我们的研究结果表明，一个家庭的细胞质mRNA或基因的肝多聚免疫球蛋白受体。三个部分测序的大鼠插入物都不相同。然而，所有三个都显示出明显的滑移同源性（见图1阴影框），这是由MACAW（4，6，7）使用的统计算法确定和绘制的。克隆2缺失了在克隆1和3中的位置7-25和1-19处以及在PolyIgR序列的位置3208-3226处发现的19-bp区段，
Our Iaboratory is interested in the control of hepatic proliferation. Recently, we described a cyclin messenger RNA (mRNA) and p32/p34CDK2’cd‘2 histone H1-kinase cascade during liver regeneration after partial hepatectomy (1). This procedure also induces patterned reexpression of fetal and neonatal genes and large numbers of proto-oncogenes (2). While screening a rat poly(A) + complementary DNA (cDNA) library from 24-hr regenerating liver (constructed by X.P. Lu in this laboratory) at slightly reduced stringency (l), with a 1,245-bp human cDNA probe encoding full-length cyclin D1 mRNA (31, we isolated three clones containing cDNA inserts of approximately 208 to 342 bp from a Stratagene Lambda Uni-ZapXR plasmid expression vector (Stratagene, La Jolla, CA) (see Fig. 1 and “Note Added in Proof ’1. Thus far, after partial DNA sequencing, none of the inserts showed significant homology to human cyclin D1 mRNA (3) (either by Harr plot or by the Multiple Alignment Construction & Analysis Workbench [MACAW procedure [41), although several d[GAl-rich regions of similarity were observed among the four cDNAs (Fig. 2). Surprisingly, an NCBI Databank BLAST Search, which scanned 81,327 DNA sequences, revealed that each rat insert contained a perfect match of 43-bp homology (shaded boxes, Fig. 2) to nucleotides 3227-3269 of the 3’-UTR of GORF1, a nearly full-length pUEX clone used recently to determine the 3,269-bp cDNA sequence of the rat polymeric IgA-receptor mRNA ( 5 ) (PolyIgR; Fig. 1). A MACAW sequence-alignment search failed to reveal significant similarity between human cyclin D1 and this receptor. However, one of the inserts (clone 1) matched GORFl perfectly along a 68-bp stretch between positions 1-68 and 3202-3269, respectively, as shown in Figure 1 (P(N) I 5 x lop2’). No cDNA sequence beyond position 3269 of the rat polymeric immunoglobulin receptor has been reported (relative sequence position 79 in Fig. 1). Consequently, the structure of the putatively untranslated 3’-UTR of the rat IgA receptor mRNA is unknown. Our results suggest a family of cytoplasmic mRNAs or genes for the hepatic polymeric immunoglobulin receptor. None of the three partially sequenced rat inserts is identical. Yet all three show significant slip-ment homologies (see shaded boxes, Fig. 1) as determined and plotted graphically by the statistical algorithm used by MACAW (4, 6, 7). Clone 2 is missing a 19-bp block that is found at positions 7-25 and 1-19 in both clones 1 and 3, and at positions 3208-3226 of the PolyIgR sequence,
DOI：
10.1073/pnas.87.6.2264
发表时间：
1990-03-01
影响因子：
11.1
作者：
KARLIN, S;ALTSCHUL, SF
通讯作者：
ALTSCHUL, SF