IMMUNOBLOT ASSAY: A RAPID AND SENSITIVE METHOD FOR IDENTIFICATION OF SALMONID FISH VIRUSES
IMMUNOBLOT ASSAY: A RAPID AND SENSITIVE METHOD FOR IDENTIFICATION OF SALMONID FISH VIRUSES
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免疫印迹测定：一种快速、灵敏的鲑鱼病毒鉴定方法
DOI：
10.7589/0090-3558-22.4.468
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
W. Schill
中科院分区：
文献类型：
--
作者：
P. McAllister;W. Schill
用免疫印迹法鉴定感染性胰腺坏死、感染性造血细胞坏死和病毒性出血性败血症病毒。将感染细胞培养上清液中的病毒抗原吸附在硝酸纤维素膜或Whatman 541滤纸上，用酶联免疫吸附试验技术进行检测。免疫印迹分析只需不到4小时，不需要特殊仪器。用细胞培养上清液测定的免疫印迹敏感性为105-106pfu/ml，用纯化的病毒测定的敏感性为0.85-4.0 ng病毒抗原。免疫印迹法检测和鉴定细胞培养液中的病毒。
An immunoblot assay was used to identify the viruses of infectious pancreatic necrosis, infectious hematopoietic necrosis, and viral hemorrhagic septicemia. Viral antigen in infected cell culture supernatant was adsorbed onto nitrocellulose membrane or Whatman 541 filter paper and detected by enzyme-linked immunosorbent assay techniques. The immunoblot assay took less than 4 hr to perform and required no special instrumentation. Assays using cell culture supernatant fluids showed immunoblot sensitivity was 105–106 PFU/ml. Assay sensitivity, determined using purified virus, is 0.85–4.0 ng of viral antigen. The immunoblot assay was used to detect and identify virus in cell culture fluids.