MOSSY CELL AXONAL PROJECTIONS TO THE DENTATE GYRUS MOLECULAR LAYER IN THE RAT HIPPOCAMPAL SLICE
MOSSY CELL AXONAL PROJECTIONS TO THE DENTATE GYRUS MOLECULAR LAYER IN THE RAT HIPPOCAMPAL SLICE
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DOI：
10.1002/hipo.450020403
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发表时间：
1992-10-01
期刊：
影响因子：
3.5
通讯作者：
SCHWARTZKROIN, PA
中科院分区：
文献类型：
--
作者：
BUCKMASTER, PS;STROWBRIDGE, BW;SCHWARTZKROIN, PA
同侧联结通路连接齿状回的不同隔颞平面。齿状门的神经元从原始细胞向内部分子层投射数百微米。作者假设，苔藓细胞，门的主要细胞类型，也局部突出到内部分子层。在400 μ m的切片内，苔藓细胞（1）在细胞内记录，同时刺激内分子层以测试逆向反应，和（2）用生物胞素标记，并用光学和电子显微镜检查轴突投射到分子层中。作者发现苔藓细胞可以被内分子层刺激逆向激活，并且在400 μ m海马切片内可以看到轴突向分子层的投射。在13的19个细胞内标记和电生理特征的苔藓细胞，侧枝可以追溯到分子层。这些结果表明，苔藓细胞有助于同侧的联合途径，也参与了当地的循环回路，影响颗粒细胞的活动。
The ipsilateral associational pathway connects different septotemporal levels of the dentate gyrus. Neurons of the dentate hilus project hundreds of micrometers from the cells of origin to the inner molecular layer. The authors hypothesized that mossy cells, the major cell type of the hilus, also project locally to the inner molecular layer. Within a 400 mum slice, mossy cells were (1) recorded intracellularly while the inner molecular layer was stimulated to test for antidromic responses, and (2) labeled with biocytin and examined with light and electron microscopy for axonal projections into the molecular layer. The authors found that mossy cells can be antidromically activated by inner molecular layer stimulation and that axonal projections to the molecular layer can be visualized within a 400 mum hippocampal slice. In 13 of 19 intracellularly labeled and electrophysiologically characterized mossy cells, collaterals could be traced into the molecular layer. These results suggest that mossy cells contribute to the ipsilateral associational pathway and also participate in local recurrent circuitry to influence granule cell activity.