Evolution of the 3R-MYB Gene Family in Plants.
Evolution of the 3R-MYB Gene Family in Plants.
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DOI：
10.1093/gbe/evx056
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发表时间：
2017-04-01
影响因子：
3.3
通讯作者：
Barbazuk WB
中科院分区：
文献类型：
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作者：
Feng G;Burleigh JG;Braun EL;Mei W;Barbazuk WB
植物3R-MYB转录因子是植物MYB超家族的一个重要亚类，但其进化历史和功能尚不清楚。我们从65种植物中鉴定了225种3R-MYB蛋白，包括藻类和陆地植物的所有主要谱系。在被子植物共同祖先之前的两个节段性复制事件产生了三个亚类的3R-MYB蛋白。在3R-MYB基因的结构域区域中发现了5个保守的内含子，它们是在植物进化过程中通过逐步增加内含子而产生的。选择物种的选择性剪接（AS）分析显示，超过60%的3R-MYB基因的转录本经历AS。AS可以通过产生不同的调控基序来调控某些植物3R-MYB的转录活性。所有亚组的3R-MYB基因似乎在其上游启动子区域内富含有丝分裂特异性激活因子元件核心序列，这表明其功能参与细胞周期。值得注意的是，来自不同物种的3R-MYB基因的表达在各种非生物胁迫下表现出差异调节。这些数据表明，植物3R-MYBs在细胞周期调控和非生物胁迫反应中起作用，这可能有助于植物适应固着生活方式。
Plant 3R-MYB transcription factors are an important subgroup of the MYB super family in plants; however, their evolutionary history and functions remain poorly understood. We identified 225 3R-MYB proteins from 65 plant species, including algae and all major lineages of land plants. Two segmental duplication events preceding the common ancestor of angiosperms have given rise to three subgroups of the 3R-MYB proteins. Five conserved introns in the domain region of the 3R-MYB genes were identified, which arose through a step-wise pattern of intron gain during plant evolution. Alternative splicing (AS) analysis of selected species revealed that transcripts from more than 60% of 3R-MYB genes undergo AS. AS could regulate transcriptional activity for some of the plant 3R-MYBs by generating different regulatory motifs. The 3R-MYB genes of all subgroups appear to be enriched for Mitosis-Specific Activator element core sequences within their upstream promoter region, which suggests a functional involvement in cell cycle. Notably, expression of 3R-MYB genes from different species exhibits differential regulation under various abiotic stresses. These data suggest that the plant 3R-MYBs function in both cell cycle regulation and abiotic stress response, which may contribute to the adaptation of plants to a sessile lifestyle.