Increased apoptosis during morphogenesis of the lower cheek teeth in tabby/EDA mice
Increased apoptosis during morphogenesis of the lower cheek teeth in tabby/EDA mice
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DOI：
10.1177/154405910508400304
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发表时间：
2005-03-01
影响因子：
7.6
通讯作者：
Peterkova, R
中科院分区：
文献类型：
--
作者：
Boran, T;Lesot, H;Peterkova, R
在野生型（WT）小鼠中，上皮细胞凋亡与胚胎牙齿数量减少和第一磨牙近中界限有关。我们研究了细胞凋亡是否也可以参与减少牙齿数量和确定异常的牙齿边界在虎斑（Ta）/EDA小鼠。利用连续的组织切片和计算机辅助三维重建，我们研究了胚胎14.5-17.5天的下颊牙列上皮细胞凋亡。与WT小鼠相比，从第15.5天起，Ta牙上皮细胞凋亡主要在近中增加。在Ta牙列的所有5种形态类型（Ia、B、c和IIa、B）中，这种凋亡显示出相似的近远中程度，并且在形态类型IIa、B中消除了第一颊齿。细胞凋亡似乎没有发挥任何因果关系的作用，在定位牙间间隙。目前的数据分析表明，Ta小鼠的细胞凋亡增加是牙齿发育受损的牙齿上皮细胞分割缺陷所造成的后果。
In wild-type (WT) mice, epithelial apoptosis is involved in reducing the embryonic tooth number and the mesial delimitation of the first molar. We investigated whether apoptosis could also be involved in the reduction of tooth number and the determination of anomalous tooth boundaries in tabby (Ta)/EDA mice. Using serial histological sections and computer-aided 3D reconstructions, we investigated epithelial apoptosis in the lower cheek dentition at embryonic days 14.5-17.5. In comparison with WT mice, apoptosis was increased mainly mesially in Ta dental epithelium from day 15.5. This apoptosis showed a similar mesio-distal extent in all 5 morphotypes ( Ia, b, c and IIa, b) of Ta dentition and eliminated the first cheek tooth in morphotypes IIa, b. Apoptosis did not appear to play any causal role in positioning inter-dental gaps. Analysis of the present data suggests that the increased apoptosis in Ta mice is a consequence of impaired tooth development caused by a defect in segmentation of dental epithelium.