Production of the Alkaloid Swainsonine by a Fungal Endosymbiont of the Ascomycete Order Chaetothyriales in the Host Ipomoea carnea
Production of the Alkaloid Swainsonine by a Fungal Endosymbiont of the Ascomycete Order Chaetothyriales in the Host Ipomoea carnea
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DOI：
10.1021/jf4008423
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发表时间：
2013-04-24
影响因子：
6.1
通讯作者：
Marcolongo-Pereira, Clairton
中科院分区：
文献类型：
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作者：
Cook, Daniel;Beaulieu, Wesley T.;Marcolongo-Pereira, Clairton
旋花科（牵牛花科）的一些植物物种来自南美洲、非洲和澳大利亚，在放牧牲畜中引起由苦马豆素引起的神经系统疾病。这些旋花科物种包括Ipomoea carnea含有吲哚里西啶生物碱苦马豆素（a-甘露糖苷酶和甘露糖苷酶II的抑制剂）和多羟基去甲托烷生物碱（糖苷酶抑制剂的calystegines）。苦马豆素已被证明是由黄芪属和棘豆属的豆科植物中的真菌内共生体产生的，在那里它在放牧牲畜中引起类似的神经系统疾病，称为疯疯癫癫。在这里，我们证明了我。用真菌内共生体感染鼠尾草植物，所述真菌内共生体是从其种子培养的，并且在纯培养物中产生苦马豆素，但不产生萼甲素。通过PCR和在I.含有苦马豆素的鼠尾草属植物。真菌内共生体属于子囊菌目毛壳菌目。来自杀真菌剂处理过的种子的植物缺乏苦马豆素，但萼甲素浓度不变。
Some plant species within the Convolvulaceae (morning glory family) from South America, Africa, and Australia cause a neurologic disease in grazing livestock caused by swainsonine. These convolvulaceous species including Ipomoea carnea contain the indolizidine alkaloid swainsonine, an inhibitor of alpha-mannosidase and mannosidase II, and polyhydroxy nortropane alkaloids, the calystegines which are glycosidase inhibitors. Swainsonine has been shown to be produced by a fungal endosymbiont in legumes of the Astragalus and Oxytropis genera, where it causes a similar neurologic disease in grazing livestock called locoism. Here we demonstrate that I. carnea plants are infected with a fungal endosymbiont that was cultured from its seeds and which produced swainsonine in pure culture but not the calystegines. The same fungal endosymbiont was detected by PCR and by culturing in I. carnea plants containing swainsonine. The fungal endosymbiont belongs to the Ascomycete order Chaetothyriales. Plants derived from fungicide-treated seeds lacked swainsonine, but calystegine concentrations were unaltered.