Identification of molecular target of diallyl trisulfide in leukemic cells
Identification of molecular target of diallyl trisulfide in leukemic cells
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DOI：
10.1080/09168451.2014.921563
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发表时间：
2014-08
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通讯作者：
Shuntaro Suda;Kazunari Watanabe;Yuki Tanaka;Kazuhiro Watanabe;Rie Tanaka;J. Ogihara;T. Ariga;Tomomi Hosono-Fukao;Takashi Hosono;T. Seki
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作者：
Shuntaro Suda;Kazunari Watanabe;Yuki Tanaka;Kazuhiro Watanabe;Rie Tanaka;J. Ogihara;T. Ariga;Tomomi Hosono-Fukao;Takashi Hosono;T. Seki
为了确定二烯丙基三硫（DATS）在人白血病细胞系U937中的分子靶点，我们通过氧化还原2D PAGE检测了细胞蛋白巯基的修饰。DATS处理后出现的一个独特的蛋白质点被鉴定为热休克蛋白27（HSP 27）。Hsp27可能是U937 DATS作用的分子靶点之一。
To identify the molecular target of diallyl trisulfide (DATS) in human leukemic cell line U937, we examined modification of thiol group(s) of cellular proteins by the redox 2D PAGE. A unique protein spot appeared by DATS treatment was identified to be heat shock protein 27 (HSP27). Hsp27 is suggested to be one of the molecular target of DATS in U937.