Membrane-type matrix metalloproteinase-1(MT1-MMP) gene is overexpressed in highly invasive hepatocellular carcinomas
Membrane-type matrix metalloproteinase-1(MT1-MMP) gene is overexpressed in highly invasive hepatocellular carcinomas
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DOI：
10.1016/0168-8278(88)80010-2
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发表时间：
1998-02-01
影响因子：
25.7
通讯作者：
Imamura, M
中科院分区：
文献类型：
--
作者：
Harada, T;Arii, S;Imamura, M
背景/目标：基质金属蛋白酶（matrix metalloproteinase，MMP）家族通过降解细胞外基质在肿瘤细胞的侵袭中发挥重要作用，本研究旨在探讨膜型基质金属蛋白酶-1（membrane-type matrix metalloproteinase-1，MT 1-MMP）在肝细胞癌中的表达模式及其与肿瘤细胞侵袭能力的关系。应用北方印迹法检测25例肝癌组织中MT 1-MMP mRNA的表达，并分析其与临床病理特征的关系。结果：北方印迹分析显示，所有病例的肿瘤组织中均有高水平的MT 1-MMP mRNA表达，而在非肿瘤组织中，有22/25例有中等或微弱的表达。25例中有21例肿瘤部位的表达高于非肿瘤部位。尤其是在有包膜浸润的肝癌中，其表达显著高于无包膜浸润的肝癌（p
Background / Aims: The matrix metalloproteinase (MMP) family play important roles in the invasion of cancer cells by degrading the extracellular matrices, The current study was designed to determine the expression pattern of membrane-type matrix metalloproteinase-1 (MT1-MMP) in hepatocellular carcinomas and its participation in invasion potential.Methods: MT1-MMP mRNA expression was examined in 25 human hepatocellular carcinoma specimens using Northern blot, and the correlation to clinicopathological features was evaluated. In situ hybridization and immunohistochemistry were performed to study the localization and the cells responsible for the production.Results: Northern blot analysis revealed high levels of MT1-MMP mRNA expression in tumorous portions in all cases, whereas in non-tumorous portions moderate or faint expression was evident in 22/25 cases. In 21/25 cases, the expression levels in tumorous portion were higher than those in non-tumorous portion. In particular, hepatocellular carcinoma with capsule infiltration emonstrated significantly higher expression than those without (p