Golgi‐impregnated amacrine cells and GABAergic retinal neurons: A comparison of dendritic, immunocytochemical, and histochemical stratification in the inner plexiform layer of rat retina
Golgi‐impregnated amacrine cells and GABAergic retinal neurons: A comparison of dendritic, immunocytochemical, and histochemical stratification in the inner plexiform layer of rat retina
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高尔基体浸渍的无长突细胞和 GABA 能视网膜神经元：大鼠视网膜内丛状层树突、免疫细胞化学和组织化学分层的比较
DOI：
10.1002/cne.901970110
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发表时间：
1981
影响因子：
2.5
通讯作者：
J. E. Vaughn
中科院分区：
文献类型：
--
作者：
E. Famiglietti;J. E. Vaughn
本文研究了谷氨酸脱羧酶（GAD）免疫细胞化学在大鼠视网膜内丛状层产生的带状图案。将这种模式与神经元的树突状分层进行了比较，树突状分层是高尔基体制剂中gaba能无突细胞的合理候选，也与其他组织化学技术产生的带状模式进行了比较。首先，定量描述了中央视网膜中5个密集的GAD阳性带和4个中间的低密度带的间距。其次，研究了高尔基浸渍无突细胞的结构细节，特别是树突层状结构。用计算机重建的这些窄域、多层的无性体的树突树与GAD阳性带模式进行了比较。这组无突神经元被认为代表了大鼠视网膜中的许多gaba能神经元，通过它们的树突形态和分层来解释GAD阳性突触终端的形式和分布。第三，通过比较几种组织化学方法的结果，得出了大鼠视网膜内丛状层的层状细分（分层）的一般模式。最后，本文指出了哺乳动物中GAD阳性无毛细胞树突分布的异同，并讨论了它们广泛分布的功能含义。在哺乳动物和某些非哺乳动物脊椎动物之间存在显著差异，其中GAD阳性树突局限于内丛状层的膜下b （ON中心细胞）。
This paper concerns the banding pattern produced in the inner plexiform layer of rat retina by glutamic acid decarboxylase (GAD) immunocytochemistry. It presents a comparison of this pattern with the dendritic stratification of neurons that are reasonable candidates for GABAergic amacrine cells in Golgi preparations, and also with the banding patterns produced by other histochemical techniques. First, the spacing of five dense GAD‐positive bands and four intervening less dense bands in central retina is quantitatively described. Second, examples of a particular, morphologically homogenous group of Golgi‐impregnated amacrine cells are examined in the details of their structure, espeically with regard to their dendritic stratification. Computer reconstructions of the dendritic trees of some of these narrow‐field, multistratified amacrines are compared with the GAD‐positive banding pattern. This group of amacrines is judged to represent many of the GABAergic neurons in rat retina, accounting for the form and distribution of GAD‐positive synaptic terminals by their dendritic morphology and stratification. Third, a general schema for the laminar subdivision (stratification) of the inner plexiform layer in rat retina is derived from a comparison of the results of several histochemical procedures. Finally, similarities and differences in the distribution of GAD‐positive amacrine cell dendrites are noted among mammals and the functional implications of their broad distribution are discussed. A conspicuous difference is cited between mammals and certain nonmammalian vertebrates in which GAD‐positive dendrites are restricted to sublamina b (ON‐center cells) of the inner plexiform layer.