Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.
Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.
复制标题
前体B细胞受体依赖性B细胞增殖和分化不需要骨髓或胎儿肝脏环境。
DOI：
10.1084/jem.191.1.23
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发表时间：
2000-01-03
期刊：
影响因子：
--
通讯作者：
Andersson J
中科院分区：
文献类型：
--
作者：
Rolink AG;Winkler T;Melchers F;Andersson J
分析了来自胎肝和骨髓的前体B（前B）I细胞在体外增殖和分化为表面免疫球蛋白阳性的未成熟B细胞的能力。胎肝和骨髓来源的祖细胞都以前B细胞受体（前BCR）依赖性方式在单独的组织培养基中这样做，而不添加其他细胞或细胞因子。大约20%的初始前BI细胞进入一次以上的分裂。单细胞水平的分析表明，大约15%的细胞分裂2到5次。前BI细胞与基质细胞的共培养不增强增殖或分化，而白细胞介素7的存在，特别是与基质细胞的组合，主要导致前BI细胞的扩增，并阻止其进一步分化。因此，前BCR不需要胎肝或骨髓的环境来发挥其功能，即选择和扩增已经经历框内VH-DHJH重排的细胞，所述重排产生前BCR相容的μH链。前BCR的配体似乎不太可能驱动这种前B细胞增殖。
The capacity of precursor B (pre-B) I cells from fetal liver and bone marrow to proliferate and differentiate into surface immunoglobulin–positive immature B cells in vitro was analyzed. Both fetal liver– and bone marrow–derived progenitors do so in a pre-B cell receptor (pre-BCR)–dependent manner in tissue culture medium alone, without addition of other cells or cytokines. Approximately 20% of the initial pre-B I cells enter more than one division. Analyses at the single-cell level show that ∼15% divide two to five times. Coculture of pre-B I cells with stromal cells did not enhance proliferation or differentiation, whereas the presence of interleukin 7, especially in combination with stromal cells, resulted mainly in the expansion of pre-B I cells and prevented their further differentiation. Thus, the environment of fetal liver or bone marrow is not required for the pre-BCR to exert its function, which is to select and expand cells that have undergone an inframe VH-DHJH rearrangement that produces a pre-BCR–compatible μH chain. It appears unlikely that a ligand for the pre-BCR drives this pre-B cell proliferation.