Marek's disease virus-induced transient paralysis is associated with cytokine gene expression in the nervous system
Marek's disease virus-induced transient paralysis is associated with cytokine gene expression in the nervous system
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DOI：
10.1089/vim.2006.19.167
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发表时间：
2006-06-01
期刊：
影响因子：
2.2
通讯作者：
Sharif, S.
中科院分区：
文献类型：
--
作者：
Abdul-Careem, M. F.;Hunter, B. D.;Sharif, S.
通过腹腔接种马立克病病毒（Marek's disease virus， MDV） RB1B株，实验诱导鸡马立克病（Marek's disease， MD）相关的短暂性麻痹（transient paralysis， TP）。在感染后7至11天（d.p.i）， 18%的感染鸡出现颈部和肢体瘫痪，并伴有不同程度的水肿、空泡形成、血管周围单个核细胞的弯曲以及主要在大脑、小脑和脑干的胶质细胞浸润。被感染但直到12 d.p.i.才发展为TP的鸡在大脑、小脑和脑干也有类似的病变，提示脑炎。与未感染的鸡相比，感染MDV的鸡脑组织中有更多的白细胞介素(IL)-6、IL-12和干扰素(IFN)- γ。此外，与未感染的鸟类相比，出现TP临床症状的鸟类脑组织中IL-18明显增加。重要的是，与无症状的mdv感染鸡相比，有TP症状的mdv感染鸡脑组织中IL-6、IL-18和ifn - γ的表达显著增加。出现TP临床症状的鸡脑内MDV基因组负荷高于无症状感染的鸡，但差异无统计学意义。在感染mdv的鸡中，颈、胸和腰椎脊髓节段的病变主要表现为血管周围单个核细胞的剪切，与组无关。TP鸡脊髓组织中IL-18和ifn - γ基因mRNA的表达与临床正常、感染和未感染的鸡相比无显著差异。这些结果提示了mdv诱导的TP可能的潜在免疫机制。
Marek's disease (MD)-associated transient paralysis (TP) was experimentally induced in chickens by intraperitoneal inoculation of RB1B strain of Marek's disease virus (MDV). Between 7 and 11 days post-infection (d.p.i.), neck and limb paralysis was observed in 18% of infected chickens, which was associated with various degrees of edema, vacuolation, perivascular cuffing of mononuclear cells, and glial cell infiltration mainly in the cerebrum, cerebellum, and brain stem. The chickens that were infected but did not progress to develop TP until 12 d.p.i. also had similar lesions suggestive of encephalitis in the cerebrum, cerebellum, and brain stem. Chickens infected with MDV had more interleukin (IL)-6, IL-12, and interferon (IFN)-gamma in their brain tissues compared to uninfected chickens. Moreover, IL-18 was significantly increased in brain tissues of birds showing clinical signs of TP compared to uninfected birds. Importantly, the expression of IL-6, IL-18, and IFN-gamma in brain tissues of MDV-infected chickens with signs of TP was significantly increased compared to that in asymptomatic MDV-infected birds. MDV genome load in the brain of chickens showing clinical signs of TP was higher than that in asymptomatic MDV-infected chickens but was not statistically significant. The lesions in the cervical, thoracic, and lumbar spinal cord segments in MDV-infected chickens were characterized mainly by perivascular cuffing of mononuclear cells irrespective of the group. The expression of mRNA for IL-18 and IFN-gamma genes was not significantly different in spinal cord tissues of chickens with TP compared to clinically normal, MDV-infected and noninfected chickens. These results suggest possible underlying immunologic mechanisms for MDV-induced TP.