LOCATION OF FILAMENTOUS PHAGE MINOR COAT PROTEINS IN PHAGE AND IN INFECTED-CELLS
LOCATION OF FILAMENTOUS PHAGE MINOR COAT PROTEINS IN PHAGE AND IN INFECTED-CELLS
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DOI：
10.1006/jmbi.1995.0393
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发表时间：
1995-07-21
影响因子：
5.6
通讯作者：
MODEL, P
中科院分区：
文献类型：
--
作者：
ENDEMANN, H;MODEL, P
我们发现，噬菌体F1的所有微小外壳蛋白在组装前都是完整的内膜蛋白，因此所有的噬菌体结构和形态发生蛋白都定位在感染细胞的膜上，这与噬菌体组装的模型是一致的，在该模型中，形态发生与噬菌体挤出一致。我们的数据表明，次要的外壳蛋白PVI和PIII已经与膜中的主要外壳蛋白PVIII联系在一起。另一方面，PVI和PIII不能以复合体的形式从膜上恢复，即使用解离的噬菌体实验表明它们在噬菌体中是相关联的。次要的外壳蛋白pVII和pviii也与噬菌体有关。通过使用针对次要蛋白的血清，我们发现次要外壳蛋白Pix可以在完整的噬菌体中获得，但PVI和pVII不能。与早期的结果一致，连接蛋白PIII清楚地暴露在噬菌体的外部。被感染的细胞含有的pvii和pix大约是并入噬菌体的十倍。
We show that all minor coat proteins of phage f1 are integral inner membrane proteins prior to assembly Hence all phage structural and morphogenetic proteins are localized in the membrane of the infected cell, consistent with models of phage assembly in which morphogenesis is coincident with phage extrusion. Our data suggest that the minor coat proteins, pVI and pIII, are already associated with the major coat protein, pVIII, in the membrane. On the other hand pVI and pIII are not recovered as a complex from the membrane, even though experiments with dissociated phage show they are associated in phage. The minor coat proteins, pVII and pVIII, are also associated in phage. With the use of sera directed against the minor proteins, we show that the minor coat protein, pIX, is accessible in intact phage, but pVI and pVII are not. Consistent with earlier results, the attachment protein, pIII clearly is exposed on the phage exterior. Infected cells contain about ten times more pVII and pIX than is incorporated into phage.