A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality.
A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality.
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DOI：
10.1016/j.cell.2018.06.035
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发表时间：
2018-07-12
期刊：
影响因子：
64.5
通讯作者：
Zeng H
中科院分区：
文献类型：
--
作者：
Daigle TL;Madisen L;Hage TA;Valley MT;Knoblich U;Larsen RS;Takeno MM;Huang L;Gu H;Larsen R;Mills M;Bosma-Moody A;Siverts LA;Walker M;Graybuck LT;Yao Z;Fong O;Nguyen TN;Garren E;Lenz GH;Chavarha M;Pendergraft J;Harrington J;Hirokawa KE;Harris JA;Nicovich PR;McGraw MJ;Ollerenshaw DR;Smith KA;Baker CA;Ting JT;Sunkin SM;Lecoq J;Lin MZ;Boyden ES;Murphy GJ;da Costa NM;Waters J;Li L;Tasic B;Zeng H
现代遗传方法在提供获取大脑中不同类型细胞和促进其功能研究方面是强大的。在这里，我们报道了大量的驱动和报告基因转基因小鼠系，包括23个新的驱动系，靶向各种皮层和皮层下细胞群，26个新的报告系，表达一系列分子工具。特别是，我们描述了TIGRE2.0转基因平台，并引入了cre依赖的报告系，使光学生理学、光遗传学和遗传定义细胞群体的稀疏标记成为可能。TIGRE2.0报告者在广泛的神经元类型中打破了单拷贝靶向插入转基因的转基因表达水平障碍，与我们的第一代TIGRE系相比，它还具有简化育种策略的额外优势。这些新型转基因细胞系极大地扩展了高精度遗传工具的范围，可用于有效地识别、监测和操纵小鼠大脑中不同的细胞类型。
Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Here we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically-defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targeted-insertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.
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影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
影响因子：
3.4
作者：
Steinecke, Andre;Hozhabri, Ellie;Taniguchi, Hiroki
通讯作者：
Taniguchi, Hiroki
菌株背景影响表达四环素反式激活蛋白的小鼠的神经毒性和行为异常。
DOI：
10.1523/jneurosci.0893-12.2012
发表时间：
2012-08-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Han HJ;Allen CC;Buchovecky CM;Yetman MJ;Born HA;Marin MA;Rodgers SP;Song BJ;Lu HC;Justice MJ;Probst FJ;Jankowsky JL
通讯作者：
Jankowsky JL
影响因子：
25
作者：
通讯作者：
--
影响因子：
48
作者：
Sando, Richard, III;Baumgaertel, Karsten;Pieraut, Simon;Torabi-Rander, Nina;Wandless, Thomas J.;Mayford, Mark;Maximov, Anton
通讯作者：
Maximov, Anton