THE HERPES-SIMPLEX VIRUS-1 RNA-BINDING PROTEIN U(S)11 IS A VIRION COMPONENT AND ASSOCIATES WITH RIBOSOMAL 60S SUBUNITS
THE HERPES-SIMPLEX VIRUS-1 RNA-BINDING PROTEIN U(S)11 IS A VIRION COMPONENT AND ASSOCIATES WITH RIBOSOMAL 60S SUBUNITS
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DOI：
10.1128/jvi.66.6.3624-3632.1992
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发表时间：
1992-06-01
影响因子：
5.4
通讯作者：
ROIZMAN, B
中科院分区：
文献类型：
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作者：
ROLLER, RJ;ROIZMAN, B
单纯疱疹病毒1u (s)11基因编码位点和构象特异性RNA结合调节蛋白。我们将该蛋白的编码序列与β -半乳糖苷酶的编码序列融合，在大肠杆菌中表达嵌合基因，并纯化出一种与被感染细胞蛋白结合RNA的融合蛋白。利用融合蛋白制备抗u (s)11单克隆抗体。对该抗体的研究表明，U(s)11蛋白是一种病毒结构蛋白，估计每个病毒粒子有600至1000个拷贝。绝大多数胞质U(s)11蛋白与感染细胞核糖体的60S亚基相关。U(s)11蛋白在其合成的感染后期和被病毒粒子引入细胞质后的感染时与核糖体结合。U(s)11蛋白在稳定转染U(s)11基因的未感染细胞系中表达，U(s)11蛋白与核糖体60S亚基相关，并定位于核核，这表明U(s)11蛋白不需要其他病毒功能来实现这些关联。
The herpes simplex virus 1 U(s)11 gene encodes a site- and conformation-specific RNA binding regulatory protein. We fused the coding sequence of this protein with that of beta-galactosidase, expressed the chimeric gene in Escherichia coli, and purified a fusion protein which binds RNA in the same way as the infected cell protein. The fusion protein was used to generate anti-U(s)11 monoclonal antibody. Studies with this antibody showed that U(s)11 protein is a viral structural protein estimated to be present in 600 to 1,000 copies per virion. The great majority of cytoplasmic U(s)11 protein was found in association with the 60S subunit of infected cell ribosomes. U(s)11 protein associates with ribosomes both late in infection at the time of its synthesis and at the time of infection after its introduction into the cytoplasm by the virion. U(s)11 protein expressed in an uninfected cell line stably transfected with the U(s)11 gene associates with ribosomal 60S subunits and localizes to nucleoli, suggesting that U(s)11 protein requires no other viral functions for these associations.