Expression of basigin, a member of the immunoglobulin superfamily, in the mouse central nervous system
Expression of basigin, a member of the immunoglobulin superfamily, in the mouse central nervous system
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DOI：
10.1016/s0168-0102(97)00119-3
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发表时间：
1998-01-01
影响因子：
2.9
通讯作者：
Kadomatsu, K
中科院分区：
文献类型：
--
作者：
Fan, QW;Yuasa, S;Kadomatsu, K
Basigin（Bsg）是属于免疫球蛋白超家族的跨膜糖蛋白。鸡Bsg（HT 7/neurothelin/5A 11）在成神经细胞中表达，但在细胞分化的特定阶段后从神经元中消失，并且在成人脑中仅在毛细血管内皮中表达。我们在此显示，通过原位杂交，Bsg mRNA的表达在神经母细胞在13.5天的小鼠胚胎。在成年小鼠中，Bsg在脑的亚区域中差异表达。在边缘系统，包括嗅觉系统，海马结构，隔区，杏仁核，丘脑前核，下丘脑，中脑被盖，内嗅皮层，扣带回中检测到Bsg的强表达。Bsg还在视网膜神经元层、大脑新皮质的Vth层、小脑的浦肯野细胞、脑干的几个核团和脊髓的灰质中强烈表达。虽然原位杂交显示，在脑毛细血管内皮细胞的弱信号，BSG的蛋白表达强到足以通过免疫组化检测。北方印迹分析证实了Bsg在中枢神经系统的强表达。考虑到Bsg基因敲除小鼠表现出行为异常，但血脑屏障功能正常，本研究结果表明，Bsg在中枢神经系统的神经元相互作用中发挥积极作用。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Basigin (Bsg) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. Chicken Bsg (HT7/neurothelin/5A11) is expressed in neuroblasts, but disappears from neurons after a specific stage of cytodifferentiation, and becomes restrictedly expressed in the capillary endothelium in the adult brain. We show herein by means of in situ hybridization that Bsg mRNA was expressed in neuroblasts in 13.5 day old mouse embryos. In the adult mouse, Bsg was differentially expressed in subregions of the brain. Strong Bsg expression was detected in the limbic system, including the olfactory system, hippocampal formation, septal area, amygdala, thalamic anterior nuclei, hypothalamus, mesencephalic tegmentum, entorhinal cortex, and cingulate gyrus. Bsg was also intensely expressed in the retinal neuronal layers, the Vth layer of the cerebral neocortex, Purkinje cells of the cerebellum, several nuclei of the brain stem, and the gray matter of the spinal cord. Although in situ hybridization showed a weak signal in the brain capillary endothelium, protein expression of Bsg was strong enough to be detected by immunohistochemistry. Northern blot analysis confirmed the strong expression of Bsg in the central nervous system. Taking into account that Bsg knockout mice exhibit abnormalities in behavior, but a normal blood-brain barrier function, the present findings suggest that Bsg functions actively in neuronal interactions in the central nervous system. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.