Fertile triploid males: an uncommon case among hybrid vertebrates.
Fertile triploid males: an uncommon case among hybrid vertebrates.
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可育的三倍体雄性：杂交脊椎动物中的罕见病例。
DOI：
10.1002/jez.363
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
V. Almada
中科院分区：
文献类型：
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作者：
C. Sousa‐Santos;M. COLLARES;V. Almada
特有的伊比利亚小鱼Squalius alburnoides是一个复杂的鱼的杂交起源，包括男性和女性具有不同的倍性水平和不同比例的父母的基因组。本文证明了与许多脊椎动物杂交谱系不同的是，苏云金杆菌三倍体杂种雄性的精子在染色体上的分布。alburnoides是可行的和功能齐全的。流式细胞术和应用于祖细胞和后代的β-肌动蛋白核基因片段的序列分析证明，这些男性产生精子克隆，如已经描述的二倍体杂交。不同类型的可育雄性（具有正常减数分裂的非杂交二倍体和二倍体和三倍体杂交种）的存在，加上雌性的杂交减数分裂，赋予这种脊椎动物复合体与其他物种的高度独立性，并有助于维持其遗传变异性。
The endemic Iberian minnow Squalius alburnoides is a complex of fishes of hybrid origin including both males and females with distinct ploidy levels and varying proportions of the parental genomes. In this paper we demonstrated that in contrast to many vertebrate hybrid lineages the sperm of triploid hybrid males of S. alburnoides is viable and fully functional. Flow cytometry and analysis of sequences of a fragment of the beta-actin nuclear gene applied to progenitors and offspring evidenced that these males produced their sperm clonally, as already described for diploid hybrids. The presence of different types of fertile males (nonhybrid diploids with normal meiosis and both diploid and triploid hybrids) coupled with hybridogenetic meiosis in females endows this vertebrate complex with a high level of independence from other species and contributes to maintain its genetic variability.