Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing
Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing
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DOI：
10.1007/978-1-4939-7306-4_23
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发表时间：
2018-01-01
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影响因子：
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通讯作者：
Kunkel, Thomas A.
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文献类型：
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作者：
Orebaugh, Clinton D.;Lujan, Scott A.;Kunkel, Thomas A.
Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequencing (HydEn-seq) to map ribonucleotides inserted into the genome of Saccharomyce cerevisiae strains deficient in ribonucleotide excision repair. We use this method to map several genomic features in wild-type and replicase variant yeast strains.
