Entry of feline calicivirus is dependent on clathrin-mediated endocytosis and acidification in endosomes
Entry of feline calicivirus is dependent on clathrin-mediated endocytosis and acidification in endosomes
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DOI：
10.1128/jvi.02452-05
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发表时间：
2006-08-01
影响因子：
5.4
通讯作者：
Brown, T. David K.
中科院分区：
文献类型：
--
作者：
Stuart, Amanda D.;Brown, T. David K.
猫杯状病毒是猫呼吸道疾病的主要病原体。它也是Caliciviridde为数不多的可栽培成员之一。我们研究了猫杯状病毒（FCV）的进入过程。早期的一项研究表明，可能需要对核内体进行酸化。我们已经证实了这一观察结果，并对其进行了扩展，证明使用药物抑制各种内吞途径和显性阴性突变体，FCV通过网格蛋白介导的内吞作用感染细胞。我们还观察到，在感染早期，FCV渗透细胞膜，使α -肌蛋白等毒素得以共同进入。内核体酸化抑制剂如氯喹和巴菲霉素A1阻断了这种渗透事件，表明酸化是基因组脱壳和进入细胞质所必需的。
Feline calicivirus is a major causative agent of respiratory disease in cats. It is also one of the few cultivatable members of Caliciviridde. We have examined the entry process of feline calicivirus (FCV). An earlier study demonstrated that acidification in endosomes may be required. We have confirmed this observation and expanded upon it, demonstrating, using drugs to inhibit the various endocytic pathways and dominant-negative mutants, that FCV infects cells via clathrin-mediated endocytosis. We have also observed that FCV permeabilizes cell membranes early during infection to allow the coentry of toxins such as alpha-sarcin. Inhibitors of endosome acidification such as chloroquine and bafilomycin A1 blocked this permeabilization event, demonstrating that acidification is required for uncoating of the genome and access to the cytoplasm.