Enhanced thermotolerance and ethanol tolerance in Saccharomyces cerevisiae mutated by high-energy pulse electron beam and protoplast fusion
Enhanced thermotolerance and ethanol tolerance in Saccharomyces cerevisiae mutated by high-energy pulse electron beam and protoplast fusion
复制标题
高能脉冲电子束和原生质体融合突变的酿酒酵母增强耐热性和乙醇耐受性
DOI：
10.1007/s00449-012-0734-0
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发表时间：
2012-11-01
影响因子：
3.8
通讯作者：
Wang, Shi-Long
中科院分区：
文献类型：
--
作者：
Zhang, Min;Xiao, Yu;Wang, Shi-Long
为了提高酿酒酵母YZ1菌株的耐热性和乙醇耐受性，研究了高能脉冲电子束（HEPE）和三轮原生质体融合的策略。YF31菌株具有耐高温、耐高乙醇、生长快、产量高的特点。YF31可以在含237.5 g L−1乙醇的培养液中生长至47°C。其中，突变菌株YF31在42℃下，从200 g葡萄糖L−1中产生94.2±4.8 g乙醇，是野生菌株YZ1产量的2.48倍。
To increase thermotolerance and ethanol tolerance in Saccharomyces cerevisiae strain YZ1, the strategies of high-energy pulse electron beam (HEPE) and three rounds of protoplast fusion were explored. The YF31 strain had the characteristics of resistant to high-temperature, high-ethanol tolerance, rapid growth and high yield. The YF31 could grow on plate cultures up to 47 °C, containing 237.5 g L−1 of ethanol. In particular, the mutant strain YF31 generated 94.2 ± 4.8 g L−1 ethanol from 200 g glucose L−1 at 42 °C, which was 2.48 times the production of the wild strain YZ1.