Sequence determinants of the folding properties of box C/D kink-turns in RNA.
Sequence determinants of the folding properties of box C/D kink-turns in RNA.
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DOI：
10.1261/rna.063453.117
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Lilley DMJ
中科院分区：
文献类型：
--
作者：
Ashraf S;Huang L;Lilley DMJ
具有不同生物学功能的扭结旋转（k-turns）之间的折叠特性有显著差异。核糖体和核糖体开关k-turn在加入金属离子时通常折叠成扭结构象，盒C/D snoRNP k-turn在这些条件下保持完全展开，尽管它们在加入L7Ae蛋白时折叠。序列元件在标准核糖体k-turn （Kt-7）和盒型C/D k-turn之间系统地交换。利用荧光共振能量转移和凝胶电泳研究了折叠。三个序列元素各自以近似相加的方式贡献了古菌Kt-7和盒C/D k-turn的不同折叠特性。生物信息学分析表明，k-turn序列进化出了与其折叠特性相适应的序列。大多数核糖体和核糖体开关的k-turn都有允许在金属离子存在时进行无辅助折叠的序列。相反，盒C/D k-turn的序列需要结合蛋白质才能驱动折叠成扭结构象，这与它们在盒C/D snoRNP装置组装中的作用一致。控制序列对折叠特性影响的规则可以应用于其他标准k-turn来预测它们的折叠特性。
Folding properties differ markedly between kink-turns (k-turns) that have different biological functions. While ribosomal and riboswitch k-turns generally fold into their kinked conformation on addition of metal ions, box C/D snoRNP k-turns remain completely unfolded under these conditions, although they fold on addition of L7Ae protein. Sequence elements have been systematically exchanged between a standard ribosomal k-turn (Kt-7) that folds on addition of metal ions, and a box C/D k-turn. Folding was studied using fluorescence resonance energy transfer and gel electrophoresis. Three sequence elements each contribute in an approximately additive manner to the different folding properties of Kt-7 and box C/D k-turns from archaea. Bioinformatic analysis indicates that k-turn sequences evolve sequences that suit their folding properties to their biological function. The majority of ribosomal and riboswitch k-turns have sequences allowing unassisted folding in response to the presence of metal ions. In contrast, box C/D k-turns have sequences that require the binding of proteins to drive folding into the kinked conformation, consistent with their role in the assembly of the box C/D snoRNP apparatus. The rules governing the influence of sequence on folding properties can be applied to other standard k-turns to predict their folding characteristics.
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DOI：
10.1126/science.1176099
发表时间：
2009-09-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bleichert F;Gagnon KT;Brown BA 2nd;Maxwell ES;Leschziner AE;Unger VM;Baserga SJ
通讯作者：
Baserga SJ
影响因子：
14.9
作者：
Huang L;Wang J;Lilley DM
通讯作者：
Lilley DM
影响因子：
3.3
作者：
Reblova, Kamila;Sponer, Judit E.;Sponer, Jiri
通讯作者：
Sponer, Jiri
影响因子：
5.3
作者：
Schultz, Annemarie;Nottrott, Stephanie;Luehrmann, Reinhard
通讯作者：
Luehrmann, Reinhard
影响因子：
4.5
作者：
Turner, B;Melcher, SE;Lilley, DMJ
通讯作者：
Lilley, DMJ