Inhibition mechanism of compound ethanol extracts from wuweizi (fructus schisandrae chinensis) on renal interstitial fibrosis in diabetic nephropathy model mice.
Inhibition mechanism of compound ethanol extracts from wuweizi (fructus schisandrae chinensis) on renal interstitial fibrosis in diabetic nephropathy model mice.
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DOI：
10.1016/s0254-6272(13)60090-4
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发表时间：
2012-12
期刊：
影响因子：
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通讯作者：
Yanqiu Zhang;Daning Zhang;Mianzhi Zhang
中科院分区：
文献类型：
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作者：
Yanqiu Zhang;Daning Zhang;Mianzhi Zhang
目的探讨五味子、川芎、牡蛎复方乙醇提取物（FRC）对链脲佐菌素（STZ）诱导的糖尿病肾病（ND）模型肾小球及肾小管间质纤维化的抑制作用及机制。 方法雄性C57BL/6小鼠27只，随机分为3组：非糖尿病组（ND）、STZ诱导糖尿病组（D）、STZ诱导糖尿病组，口服5 g·kg−1·day−1 FRC灌胃（DFRC），每组9只。采用Western blotting法检测肾组织中E-钙粘蛋白、α-平滑肌肌动蛋白（α-SMA）、纤溶酶原激活物抑制剂-1（PAI-1）的蛋白表达。免疫组化法检测纤连蛋白（FN）、α-SMA的表达。显微镜下观察肾组织形态学变化。结果DFRC组肾组织纤维化程度减轻。同时，DFRC组FN、α-SMA和PAI-1的表达均显着低于D组（均P<0.05）。结论FRC可以改善C57BL/6小鼠的DN，其机制可能与抑制上皮间质转分化、内皮肌成纤维细胞转化和PAI-1表达有关。
OBJECTIVETo evaluate inhibition effect and mechanism of compound ethanol extracts from Wuweizi (Fructus Schisandrae Chinensis), Chuanxiong (Rhizoma Chuanxiong) and Muli (Cocha Ostreae) (FRC) on glomerular and tubular interstitial fibrosis in streptozocin (STZ)-induced diabetic nephropathy (ND) model mice.METHODSTwenty-seven male C57BL/6 mice were divided randomly into 3 groups: nondibetic (ND), STZ-induced diabetic (D), and STZ-induced diabetic that were treated with 5 g · kg−1· day−1of FRC by oral gavage (DFRC), with 9 in each group. The protein expressions of E-cadherin, α-smooth muscle actin (α-SMA), Plasminogen Activator Inhibitor-1 (PAI-1) in renal tissues were investigated by Western blotting. The expressions of fibronectin (FN) and α-SMA were detected by immunohistochemical method. The morphological changes of renal tissues were observed under a microscope.RESULTSRenal tissues in the DFRCgroup showed a lessened degree of fibrosis. Meanwhile, the expressions of FN, α-SMA and PAI-1 were significantly lower in the DFRCgroup than those in the D group (all P<0.05).CONCLUSIONFRC can ameliorate the DN in the C57BL/6 mice, and its mechanism may relate to inhibition on the epithelial to mesenchymal transdifferentiation, endothelial-myofibroblast transition and PAI-1 expression.