Involvement of mitochondrial ferredoxin and Cox15p in hydroxylation of heme O
Involvement of mitochondrial ferredoxin and Cox15p in hydroxylation of heme O
复制标题
DOI：
10.1016/s0014-5793(01)02249-9
复制
发表时间：
2001-03-09
期刊：
影响因子：
3.5
通讯作者：
Tzagoloff, A
中科院分区：
文献类型：
--
作者：
Barros, MH;Carlson, CG;Tzagoloff, A
Cox15p是细胞色素氧化酶[Glerum et at]的生物生成所必需的。， J，生物学。化学，272(1997)19088-19094]。我们在这里表明，cox15突变体阻断血红素A的生物合成，但不阻断血红素O的生物合成。在裂糖菌中，pombe COX15与YAH1融合，YAH1是产生线粒体铁氧化还蛋白（肾上腺素还蛋白）的酵母基因。在酿酒酵母中，Cox15p和Yah1p的融合挽救了cox15和yah1零突变体，这表明Yah1p与Cox15p协同起作用。我们提出Cox15p与Yah1p及其推定的还原酶（Arh1p）一起在血红素o的羟基化中起作用。(C) 2001年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
Cox15p is essential for the biogenesis of cytochrome oxidase [Glerum et at., J, Biol. Chem, 272 (1997) 19088-19094]. We show here that cox15 mutants are blocked in heme A but not heme O biosynthesis. In Schizosaccharomyces pombe COX15 is fused to YAH1, the yeast gene for mitochondrial ferredoxin (adrenodoxin). A fusion of Cox15p and Yah1p in Saccharomyces cerevisiae rescued both cox15 and yah1 null mutants, This suggests that Yah1p functions in concert with Cox15p. We propose that Cox15p functions together with Yah1p and its putative reductase (Arh1p) in the hydroxylation of heme O. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.