Intermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
Intermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
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DOI：
10.1007/s10047-019-01141-3
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发表时间：
2019-10-15
影响因子：
1.3
通讯作者：
Shimizu, Tatsuya
中科院分区：
文献类型：
--
作者：
Sano, Kazunori;Homma, Jun;Shimizu, Tatsuya
通过血管灌流介质可以保存供体器官和培养生物工程器官。然而，由于灌注不足造成的组织损伤仍然是一个问题。我们评估了间歇外加压是否会改善培养中器官的灌注量和存活率。采用生物反应器系统灌流和培养大鼠小肠和股骨肌标本。对20名S施加间歇正压(10毫米汞柱)，每隔20分钟对S施加正压。间歇加压可使灌流20h后的小肠制剂灌流均匀，组织损伤最小，而非加压(对照)制剂的上表面灌注率明显低于下表面灌注率，组织学证据也明显低于对照组。长期研究是在表达荧光素酶的大鼠股骨肌肉制剂中进行的。与非加压对照组相比，间歇加压在整个14天的实验期内导致了更好的血流灌注，通过灌流荧光素后更高的生物发光强度表明改善了器官的存活率，并通过更好地保存血管结构和骨骼肌核来减少组织坏死水平(组织学分析)。因此，与对照组相比，间歇性施加外部正压改善了小肠和骨骼肌制剂的灌注量，并提高了组织活力。我们期望这种创新的灌流技术可以用于改善供体器官的保存和生物工程器官的培养。
The perfusion of medium through blood vessels allows the preservation of donor organs and culture of bioengineered organs. However, tissue damage due to inadequate perfusion remains a problem. We evaluated whether intermittent external pressurization would improve the perfusion and viability of organs in culture. A bioreactor system was used to perfuse and culture rat small intestine and femoral muscle preparations. Intermittent positive external pressure (10 mmHg) was applied for 20 s at intervals of 20 s. Intermittent pressurization resulted in uniform perfusion of small intestine preparations and minimal tissue damage after 20 h of perfusion, whereas non-pressurized (control) preparations exhibited significantly worse perfusion of the upper surface than the lower surface and histologic evidence of tissue damage. Longer term studies were undertaken in luciferase-expressing rat femoral muscle preparations. Compared with non-pressurized controls, intermittent pressurization led to better perfusion throughout the 14-day experimental period, improved organ viability as indicated by a higher bioluminescence intensity after perfusion with luciferin, and reduced levels of tissue necrosis with better preservation of vascular structures and skeletal muscle nuclei (histologic analyses). Therefore, intermittent application of external positive pressure improved the perfusion of small intestine and skeletal muscle preparations and enhanced tissue viability when compared with controls. We anticipate that this innovative perfusion technique could be used to improve the preservation of donor organs and culture of bioengineered organs.