Ultrastructure of the corneal nerves after fixation with potassium permanganate
Ultrastructure of the corneal nerves after fixation with potassium permanganate
复制标题
高锰酸钾固定后角膜神经的超微结构
DOI：
10.1002/ar.1091900406
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
A. Palkama
中科院分区：
文献类型：
--
作者：
T. Tervo;A. Palkama
电镜下观察KMnO_4固定后大鼠角膜的神经支配。有髓神经纤维仅在角膜缘观察到，而位于无血管角膜基质中的轴突被雪旺细胞胞质包围。在角膜所有部分的非囊泡纤维中观察到具有小（300 - 500 A）颗粒囊泡的轴突轮廓和具有无颗粒囊泡的另一种类型。上级颈神经节切除术后，不可能找到任何轴突与小颗粒囊泡。另一方面，在神经节切除后的基质神经干中观察到一些变性的轴突轮廓。在上皮中-裸露的轴突，偶尔有轴突和一些无颗粒囊泡穿透上皮细胞之间。此外，在上皮中很少观察到充满几个线粒体的轴突，但这些轴突难以与周围的上皮细胞区分开来。
The innervation of the rat cornea was investigated electron microscopically after KMnO4 fixation. Myelinated nerve fibres were observed only in the limbal margin of the cornea, whereas the axons located in the stroma of the avascular cornea were surrounded by the Schwann cell cytoplasm. Axon profiles with small (300‐500 A) granular vesicles and another type with agranular vesicles were seen among the non‐vesiculated fibres in all parts of the cornea. After superior cervical ganglionectomy it was not possible to find any axons with small granular vesicles. On the other hand, some degenerating axon profiles were observed in the stromal nerve trunks after ganglionectomy. In the epithelium‐naked axons with an occasional mitochondrion and a few agranular vesicles penetrated between the epithelial cells. Moreover, axons filled with several mito‐chondria were rarely observed in the epithelium, but these were difficult to differentiate from the surrounding epithelial cells.