MEC-7 IS A BETA-TUBULIN GENE REQUIRED FOR THE PRODUCTION OF 15-PROTOFILAMENT MICROTUBULES IN CAENORHABDITIS-ELEGANS
MEC-7 IS A BETA-TUBULIN GENE REQUIRED FOR THE PRODUCTION OF 15-PROTOFILAMENT MICROTUBULES IN CAENORHABDITIS-ELEGANS
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DOI：
10.1101/gad.3.6.870
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发表时间：
1989-06-01
影响因子：
10.5
通讯作者：
CHALFIE, M
中科院分区：
文献类型：
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作者：
SAVAGE, C;HAMELIN, M;CHALFIE, M
在秀丽隐杆线虫中，具有15条原丝的微管是6个触觉感受神经元的特化特征; elegans神经元有11个原丝。mec-7基因的突变导致了触觉不敏感的动物，它们的触觉细胞缺乏15-原丝微管。我们已经确定了mec-7基因中的54个突变。mec-7活性的缺失选择性地导致触觉敏感性的隐性丧失。部分功能丧失等位基因导致触摸灵敏度的部分丧失。显性突变以异常高的比例分离，可能编码异常产物。我们克隆了mec-7基因;它编码β-微管蛋白，其与脊椎动物β-微管蛋白90-93%相同。微管蛋白我们的研究结果是一致的假设，微管蛋白的异质性有助于形成结构和功能不同的微管。
In the namatode Caenorhabditis elegans, microtubules with 15 protofilaments are a specialized feature of six touch-receptor neurons; microtubules found in other C. elegans neurons have 11 protofilaments. Mutations in the gene mec-7 result in touch-insensitive animals whose touch cells lack the 15-protofilament microtubules. We have characterized 54 mutations in the mec-7 gene. The absence of mec-7 activity results selectively in the recessive loss of touch sensitivity. Partial loss-of-function alleles result in a partial loss of touch sensitivity. Dominant mutations, which are isolated at an unusually high proportion, may encode abnormal products. We have cloned the mec-7 gene; it encodes a .beta.-tubulin which is 90-93% identical to vertebrate .beta.-tubulin. Our results are consistent with the hypothesis that tubulin heterogeneity contributes to the formation of structurally and functionally distinct sets of microtubules.