Origins of circulating endothelial cells and endothelial outgrowth from blood
Origins of circulating endothelial cells and endothelial outgrowth from blood
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DOI：
10.1172/jci8071
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发表时间：
2000-01-01
影响因子：
15.9
通讯作者：
Hebbel, RP
中科院分区：
文献类型：
--
作者：
Lin, Y;Weisdorf, D;Hebbel, RP
正常成人外周血中有少量的循环内皮细胞（CEC），并且从血液培养物中观察到内皮生长。在这项研究中，我们寻求深入了解CEC的起源和内皮生长的血液培养。荧光原位杂交分析5-20个月前接受性别不匹配移植的骨髓移植受者的血液样本，发现新鲜血液中大多数CEC具有受体基因型。培养9天后（5倍扩增），来自相同血液样本的内皮生长仍然主要是受体基因型。相比之下，内皮生长后，类似1个月（102倍扩增），主要是供体基因型。因此，在此期间，供体基因型内皮细胞仅扩增了20倍，而供体基因型内皮细胞扩增了1000倍。这些数据表明，新鲜血液中的大多数CEC来源于血管壁，并且具有有限的生长能力。相反，数据表明来自血液培养物的内皮细胞的生长主要来源于可移植的骨髓来源的细胞。因为这些细胞有更多的延迟生长，但更大的增殖率，我们的数据表明，他们来自循环成血管细胞。
Normal adults have a small number of circulating endothelial cells (CEC) in peripheral blood, and endothelial outgrowth has been observed from cultures of blood. In this study we seek insight into the origins of CEC and endothelial outgrowth from cultures of blood. Fluorescence in situ hybridization analysis of blood samples from bone marrow transplant recipients who had received gender-mismatched transplants 5-20 months earlier showed that most CEC in fresh blood had recipient genotype. Endothelial outgrowth from the same blood samples after 9 days in culture (5-fold expansion) was still predominantly of the recipient genotype. In contrast, endothelial outgrowth after similar to 1 month (102-fold expansion) was mostly of donor genotype. Thus, recipient-genotype endothelial cells expanded only similar to 20-fold over this period, whereas donor-genotype endothelial cells expanded similar to 1000-fold. These data suggest that most CEC in fresh blood originate from vessel walls and have limited growth capability. Conversely the data indicate that outgrowth of endothelial cells from cultures of blood is mostly derived from transplantable marrow-derived cells. Because these cells have more delayed outgrowth but a greater proliferative rate, our data suggest that they are derived from circulating angioblasts.