An effective real-time colorimeteric sensor for sensitive and selective detection of cysteine under physiological conditions.
An effective real-time colorimeteric sensor for sensitive and selective detection of cysteine under physiological conditions.
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DOI：
10.1039/c0an00943a
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Zhengquan Yan;Shanyi Guang;Hongyao Xu;Xiang‐Yang Liu
中科院分区：
文献类型：
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作者：
Zhengquan Yan;Shanyi Guang;Hongyao Xu;Xiang‐Yang Liu
本文报道了一种新型的、实时的、灵敏的半胱氨酸比色传感器--二-N-甲基-N-羟乙基苯胺方酸（SQ）的合成及其在中性水溶液（pH = 7.5）中的ΔA分析。该方法用于合成氨基酸样品和人血清样品的分析。结果表明，在pH = 7.5的水介质中，半胱氨酸的线性范围为10~700 nmol L（-1），相关系数R = 0.9984，检出限（3σ，n = 20）为3.9 nmol L（-1）。半胱氨酸测定的相对标准偏差（RSD）小于4.1%（n = 5）。该方法具有简便、快速、选择性高、灵敏度高等优点。这使得第一次在正常生理条件下实时检测半胱氨酸成为可能。
In this contribution, a new, real-time and sensitive colorimetric sensor, di-N-methyl-N-hydroxyethylaniline squaraine (SQ), has been identified and synthesized for cysteine analysis based on its ΔA in neutral aqueous medium (pH ≈ 7.5). The proposed method was applied to analyse synthetic amino acid samples and human serum samples. The results show that the linear range of cysteine detection in aqueous medium at pH ≈ 7.5 is 10~700 nmol L(-1) with a correlation coefficient (R) of 0.9984 and a limit of detection (3σ, n = 20) of 3.9 nmol L(-1). The relative standard deviation (RSD) for cysteine detection was lower than 4.1% (n = 5). The proposed method possesses the advantages of simplicity, rapidity, high selectivity and sensitivity. This makes it possible, for the first time, the real-time detection of cysteine under normal physiological conditions.