D-Dopachrome tautomerase is a candidate for key proteins to protect the rat liver damaged by carbon tetrachloride
D-Dopachrome tautomerase is a candidate for key proteins to protect the rat liver damaged by carbon tetrachloride
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DOI：
10.1016/j.tox.2008.09.016
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发表时间：
2009-01-08
期刊：
影响因子：
4.5
通讯作者：
Arisawa, Kokichi
中科院分区：
文献类型：
--
作者：
Hiyoshi, Mineyoshi;Konishi, Hiroaki;Arisawa, Kokichi
已知四氯化碳（CCl 4）会引起肝损伤。CCl 4注射的动物实验揭示了许多发现，特别是肝损伤和肝再生的机制。最近蛋白质组学方法已被引入各种研究中，以评估综合蛋白质组水平的定量和定性变化。本研究旨在利用蛋白质组学技术阐明肝损伤、肝保护和肝再生的关键蛋白。以4 ml/kg体重的剂量向成年雄性大鼠腹腔内给予50%（V/V）CCl 4玉米油溶液。注射后约24 h，取出肝脏，用可裂解同位素编码亲和标签（cICAT）试剂、二维凝胶电泳（2-DE）和质谱（MS）分析提取的蛋白质。表明D-多巴色素互变异构酶（DDT）增加了12倍。据报道，这种酶参与黑色素（一种抗氧化剂）的生物合成。根据组织学分析，黑色素水平增加，在未受损的肝细胞的四氯化碳处理的大鼠。这些结果表明，DDT的增加是对肝损伤的反应，加速黑色素的生物合成，并保护肝脏免受CCl 4诱导的氧化应激。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Carbon tetrachloride (CCl4) is known to induce liver damage. Animal experiments with CCl4 injections have revealed many findings, especially mechanisms of liver damage and liver regeneration. Recently. proteomic approaches have been introduced in various studies to evaluate the quantitative and qualitative changes in the comprehensive proteome level. The aim of this research is to elucidate the key protein for liver damage, liver protection and liver regeneration by using proteomic techniques. 50% (V/V) CCl4 in corn oil was administered intraperitoneally to adult male rats at a dose of 4 ml/kg body weight. Approximately 24 h after the injection, the liver was removed and extracted proteins were analyzed with cleavable isotope coded affinity tag (cICAT) reagents, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). A twelvefold increase in D-dopachrome tautomerase (DDT) was indicated. This enzyme has been reported to be involved in the biosynthesis of melanin, an antioxidant. According to the histological analysis, melanin levels were increased in un-damaged hepatocytes of CCl4-treated rats. These results suggest that the increase in DDT is a response to liver damage, accelerates melanin biosynthesis and protects the liver from oxidative stress induced by CCl4. (C) 2008 Elsevier Ireland Ltd. All rights reserved.