Effects of transforming growth factor-beta (TGF-β1) on satellite cell activation and survival during oxidative stress.
Effects of transforming growth factor-beta (TGF-β1) on satellite cell activation and survival during oxidative stress.
复制标题
转化生长因子-β (TGF-β1) 对氧化应激期间卫星细胞活化和存活的影响。
DOI：
10.1007/s10974-011-9255-8
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发表时间：
2011
影响因子：
2.7
通讯作者：
Allen,RonaldE
中科院分区：
文献类型：
--
作者：
Rathbone,ChristopherR;Yamanouchi,Keitaro;Chen,XiaoyuK;Nevoret-Bell,CedrineJ;Rhoads,RobertP;Allen,RonaldE
成人骨骼肌修复和再生的调节主要是由于称为卫星细胞的常驻成人肌源性前体细胞的贡献。在它们参与肌肉修复之前的事件包括激活（从静止退出）、增殖和分化。本研究检测了转化生长因子-β 1（TGF-β1）对卫星细胞活化的影响，确定了TGF-β1是否可以在肝细胞生长因子（HGF）存在下维持静止，以及TGF-β1对卫星细胞活化的调节是否提高了卫星细胞抵抗氧化应激的能力。在早期卫星细胞活化（0-48 h）或增殖期（48-96 h）加入TGF-β1分别维持和诱导卫星细胞静止，如通过肌源性分化（MyoD）蛋白表达所确定的。当与HGF一起使用时，TGF-β1也会减弱卫星细胞的激活。最后，研究了静止在保护细胞免受氧化应激中的作用。TGF-β1处理和低pH卫星细胞制备程序（一种在体外预先阻止自发活化的技术）均提高了过氧化氢处理后培养的卫星细胞的存活率。这些结果表明，TGF-β1能够维持和诱导卫星细胞静止，并建议维持卫星细胞静止的方法可能会提高其移植效率。
The regulation of adult skeletal muscle repair and regeneration is largely due to the contribution of resident adult myogenic precursor cells called satellite cells. The events preceding their participation in muscle repair include activation (exit from quiescence), proliferation, and differentiation. This study examined the effects of transforming growth factor-beta (TGF-β1) on satellite cell activation, determined whether TGF-β1 could maintain quiescence in the presence of hepatocyte growth factor (HGF), and whether the regulation of satellite cell activation with TGF-β1 improves the ability of satellite cells to withstand oxidative stress. The addition of TGF-β1 during early satellite cell activation (0–48 h) or during the proliferative phase (48–96 h) maintained and induced satellite cell quiescence, respectively, as determined by myogenic differentiation (MyoD) protein expression. TGF-β1 also attenuated satellite cell activation when used with HGF. Finally, the role of quiescence in protecting cells against oxidative stress was examined. TGF-β1 treatment and the low pH satellite cell preparation procedure, a technique that forestalls spontaneous activation in vitro, both enhanced survival of cultured satellite cells following hydrogen peroxide treatment. These findings indicate that TGF-β1 is capable of maintaining and inducing satellite cell quiescence and suggest methods to maintain satellite cell quiescence may improve their transplantation efficiency.