Protein-polymer nanoparticles for nonviral gene delivery.
Protein-polymer nanoparticles for nonviral gene delivery.
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DOI：
10.1021/bm101354a
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发表时间：
2011-02
影响因子：
6.2
通讯作者：
Jianjun Zhang;Y. Lei;Anandika Dhaliwal;Quinn K. T. Ng;Juanjuan Du;Ming Yan;Yunfeng Lu;T. Segura
中科院分区：
文献类型：
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作者：
Jianjun Zhang;Y. Lei;Anandika Dhaliwal;Quinn K. T. Ng;Juanjuan Du;Ming Yan;Yunfeng Lu;T. Segura
蛋白质-聚合物结合物作为非病毒基因递送载体被研究。以牛血清白蛋白为大分子引发剂，采用原位原子转移自由基聚合法制备了牛血清白蛋白-聚甲基丙烯酸二甲氨基乙酯纳米粒(NBSA)。NBSA的粒径和电荷分别为5~15 nm和+8.9~+22.5，是ATRP反应时间的函数。NBSA能凝聚质粒DNA，形成平均直径为50 nm的多聚体。与线形和分支PEI相比，nBSA/PDNA多聚体具有相似或更好的效率。有趣的是，nBSA的粒径和电荷对PDNA的络合和转基因表达没有影响，这表明在较低的电荷比下可以获得相同的基因传递效率。我们相信，通过使用蛋白质-聚合物偶联物，可以通过使用不同的蛋白质核心将额外的功能引入到多聚体中，因此，它们为非病毒基因传递载体的设计提供了一个有趣的替代方案。
Protein-polymer conjugates were investigated as nonviral gene delivery vectors. BSA-poly(dimethylamino) ethyl methacrylate (PDMA) nanoparticles (nBSA) were synthesized using in situ atom transfer radical polymerization (in situ ATRP) and BSA as a macroinitiator. The diameter and charge of nBSA was a function of the ATRP reaction time and ranged from 5 to 15 nm and +8.9 to +22.5, respectively. nBSA were able to condense plasmid DNA (pDNA) and form polyplexes with an average diameter of 50 nm. nBSA/pDNA polyplexes transfected cells with similar efficiencies or better as compared to linear and branched PEI. Interestingly, the nBSA particle diameter and charge did not affect pDNA complexation and transgene expression, indicating that the same gene delivery efficiency can be achieved with lower charge ratios. We believe that with the use of protein-polymer conjugates additional functionality could be introduced to polyplexes by using different protein cores and, thus, they pose an interesting alternative to the design of nonviral gene delivery vectors.