Antiangiogenic activity in tears: Presence of pigment-epithelium-derived factor
Antiangiogenic activity in tears: Presence of pigment-epithelium-derived factor
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DOI：
10.1159/000111153
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Van Setten, G.
中科院分区：
文献类型：
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作者：
Abdiu, O.;Van Setten, G.
背景/目的：色素上皮衍生因子(PEDF)在预防视网膜新生血管形成中具有重要作用。最近发现它存在于角膜和结膜组织中，到目前为止还没有关于它在泪液中存在的报道。基于对稀释的泪液样本的分析，初步结果甚至表明人类泪液中不存在这种因素。考虑到PEDF作为角膜血管生成调节剂的临床重要性，我们研究了未稀释的人泪液。方法：采集18例健康体检者的血清标本，用商品化的酶联免疫吸附试验进行分析。同时采集了5例翼状胬肉患者和2例视网膜脱离术后患者的视网膜下液作为阳性对照。结果：PEDF浓度均低于检出限(P<0.05)。
Background/Purpose: Pigment-epithelium-derived factor (PEDF) is of major importance to prevent neovascularization of the retina. Recently found to occur in the cornea and conjunctival tissue, its presence in tears has this far not been reported. Initial results, based on the analysis of diluted tear fluid samples, even indicated the absence of this factor in human tears. Considering the clinical importance of PEDF as regulator of corneal vascularization, we investigated undiluted human tears. Methods: Samples of 18 healthy individuals were collected and analyzed using a commercial ELISA. Samples were also collected from 5 patients with pterygium and as a positive control 2 samples of subretinal fluid from patients following retinal detachment surgery. Results: PEDF concentrations were below the detection limit (i.e.