Co-expression of mannose-ligand and non-nuclear progesterone receptors on motile human sperm identifies an acrosome-reaction inducible subpopulation.
Co-expression of mannose-ligand and non-nuclear progesterone receptors on motile human sperm identifies an acrosome-reaction inducible subpopulation.
复制标题
甘露糖配体和非核黄体酮受体在活动的人类精子上的共表达鉴定出顶体反应诱导亚群。
DOI：
10.1111/j.1600-0897.1995.tb00925.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hershlag,A
中科院分区：
文献类型：
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作者：
Benoff,S;Rushbrook,JI;Hurley,IR;Mandel,FS;Barcia,M;Cooper,GW;Hershlag,A
问题：确定孕酮和甘露糖受体的表面表达是否可以用于识别精子在带结合后可能发生顶体反应，并将这些受体的反应性与天然存在的精子头部定向抗精子抗体（asa）进行比较。方法：用细胞外孕酮观察新鲜和有能力活动的人精子表面孕酮结合位点与顶体状态的关系。比较游离黄体酮和/或甘露糖配体的精子结合率和诱导顶体反应的能力。用黄体酮-辣根过氧化物酶检测定位于质膜的精子蛋白和血清asa被动转移后沉淀的表面蛋白的Western blots。研究了相同的asa对配体结合和诱导顶体反应的影响。结果：这两个受体位于有能力运动精子的一个子集上，并且在胞外作用之前协调地从顶体帽上的质膜上清除。然而，每种配体的功能结合位点的表面外观受到不同机制的调节，黄体酮受体单独被asa特异性沉淀。asa被动转移到有能力的精子中，选择性地抑制孕酮刺激的顶体反应，但不抑制离子霉素诱导的顶体反应或精子结合甘露糖配体的能力。结论：基于这些受体的共表达或完全缺失，在体外使能条件下培养的可育供体精子可细分为顶体反应诱导亚群和不可诱导亚群。综上所述，精子表面孕酮受体与asa的反应导致了抗精子免疫不育的顶体反应不足。
PROBLEM: To determine whether surface expression of receptors for progesterone and mannose can be used to identify spermatozoa likely to undergo an acrosome reaction after zona binding and to compare the reactivity of these receptors with naturally occurring sperm head‐directed anti‐sperm antibodies (ASAs).METHOD: Progesterone binding sites on the surface of fresh and capacitated motile human sperm in relation to acrosome status were visualized using a cell‐impermeant progesterone. Free progesterone and/or mannose ligands were compared for percent sperm binding and ability to induce an acrosome reaction. Western blots of sperm proteins localized to the plasma membrane and surface proteins precipitated following passive transfer of serum ASAs were probed with progesterone‐horseradish peroxidase. The effects of the same ASAs on ligand binding and on the induced acrosome reaction were examined.RESULTS: The two receptors are located in close proximity on a subset of capacitated motile sperm and are coordinately cleared from the plasma membrane overlying the acrosomal cap prior to exocytosis. The surface appearance of functional binding sites for each ligand, however, is regulated by different mechanisms and the progesterone receptor alone is specifically precipitated by ASAs. Passive transfer of ASAs to capacitated sperm selectively inhibits the progesterone‐stimulated acrosome reaction but not the ionomycin‐induced acrosome reaction or the ability of sperm to bind mannose ligands.CONCLUSIONS: Sperm from fertile donors incubated under capacitating conditions in vitro can be subdivided into acrosome reaction inducible and noninducible subpopulations on the basis of the co‐expression or total absence of these receptors. The combined data indicate that reaction of sperm surface progesterone receptors with ASAs contributes to the acrosome reaction insufficiency observed in anti‐sperm immune infertility.