Comparative Study of Antioxidant Properties and Total Phenolic Content of 30 Plant Extracts of Industrial Interest Using DPPH, ABTS, FRAP, SOD, and ORAC Assays
Comparative Study of Antioxidant Properties and Total Phenolic Content of 30 Plant Extracts of Industrial Interest Using DPPH, ABTS, FRAP, SOD, and ORAC Assays
复制标题
DOI：
10.1021/jf803011r
复制
发表时间：
2009-03-11
影响因子：
6.1
通讯作者：
Merillon, Jean-Michel
中科院分区：
文献类型：
--
作者：
Dudonne, Stephanie;Vitrac, Xavier;Merillon, Jean-Michel
采用DPPH自由基和ABTS自由基清除能力、ORAC自由基吸收能力、超氧化物歧化酶（SOD）和铁还原抗氧化能力（FRAP）等方法，对30种植物水提液的抗氧化活性进行了研究。还通过Folin-Ciocalteu方法测定总酚含量。抗氧化性能和总酚含量在所选植物之间存在显着差异。结果发现，橡树（栎），松树（松树），肉桂（肉桂）水提取物具有最高的抗氧化能力，在大多数使用的方法，因此可能是潜在的丰富的天然抗氧化剂的来源。这些提取物呈现出最高的酚含量（300-400 mg GAE/g）。巴拉圭冬青（Ilex paraguariensis）和丁香（Eugenia caryophyllus克洛维斯）水提物也显示出强的抗氧化特性和高酚含量（约200 mg GAE/g）。一个显着的抗氧化能力和总酚含量之间的关系被发现，表明酚类化合物是这些植物的抗氧化性能的主要贡献者。
Aqueous extracts of 30 plants were investigated for their antioxidant properties using DPPH and ABTS radical scavenging capacity assay, oxygen radical absorbance capacity (ORAC) assay, superoxide dismutase (SOD) assay, and ferric reducing antioxidant potential (FRAP) assay. Total phenolic content was also determined by the Folin-Ciocalteu method. Antioxidant properties and total phenolic content differed significantly among selected plants. It was found that oak (Quercus robur), pine (Pinus maritima), and cinnamon (Cinnamomum zeylanicum) aqueous extracts possessed the highest antioxidant capacities in most of the methods used, and thus could be potential rich sources of natural antioxidants. These extracts presented the highest phenolic content (300-400 mg GAE/g). Mate (Ilex paraguariensis) and clove (Eugenia caryophyllus clovis) aqueous extracts also showed strong antioxidant properties and a high phenolic content (about 200 mg GAE/g). A significant relationship between antioxidant capacity and total phenolic content was found, indicating that phenolic compounds are the major contributors to the antioxidant properties of these plants.