Dramatic improvement in genome assembly achieved using doubled-haploid genomes.
Dramatic improvement in genome assembly achieved using doubled-haploid genomes.
复制标题
使用双单倍体基因组实现了基因组组装的显着改进。
DOI：
10.1038/srep06780
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发表时间：
2014-10-27
影响因子：
4.6
通讯作者：
Asakawa S
中科院分区：
文献类型：
--
作者：
Zhang H;Tan E;Suzuki Y;Hirose Y;Kinoshita S;Okano H;Kudoh J;Shimizu A;Saito K;Watabe S;Asakawa S
大基因组的从头组装的改进仍然是期望的。在这里，我们通过采用双单倍体个体来提高基因组序列草图的质量。我们测序野生型和双单倍体红鳍东方鲀基因组，在相同的条件下，使用Illumina平台和组装重叠群与SOAPDenovo 2。与野生型相比，我们观察到双单倍体个体的N50重叠群和支架的大小分别提高了5.4倍和2.6倍，这表明使用双单倍体基因组有助于准确的基因组分析。
Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 contig and scaffold of doubled-haploid individuals, respectively, compared to the wildtype, indicating that the use of a doubled-haploid genome aids in accurate genome analysis.