Determinant specificities of the groups B and C polysaccharides of Neisseria meningitidis.
Determinant specificities of the groups B and C polysaccharides of Neisseria meningitidis.
复制标题
脑膜炎奈瑟菌 B 组和 C 组多糖的决定性特异性。
DOI：
10.4049/jimmunol.134.4.2651
复制
发表时间：
1985
影响因子：
4.4
通讯作者：
F. Michon
中科院分区：
文献类型：
--
作者：
H. Jennings;R. Roy;F. Michon
B群脑膜炎球菌特异性马抗血清含有至少两种不同的抗体群，其对B群荚膜多糖抗原上的决定簇具有特异性。这两个群体的基础上进行了区分，只有其中一个被吸收的抗血清的结构相关的colominic酸的能力。利用一系列线性α-（2-----8）-连接的唾液酸寡聚体作为抑制剂，通过放射免疫抑制技术阐明了Colominic酸特异性决定簇的性质。通过预先除去其N-乙酰基，然后用[3 H]乙酸酐取代来标记可龙酸。的构象性质的决定提出，因为不寻常的大尺寸（10个唾液酸残基）的低聚物需要作为一个有效的抑制剂。负责第二群B组特异性抗体的决定簇的结构尚未确定，但显然是基于B组脑膜炎球菌多糖特有的尚未确定的构象或结构特征。与结肠菌酸特异性B组决定簇相反，负责C组多糖特异性兔抗体的决定簇被证明是更常规的。α-（2-9）-连接的寡聚体的抑制特性在含有4个或5个唾液酸残基的寡聚体中最大化，这与抗体位点的近似估计最大大小一致。
A meningococcal group B-specific horse antiserum contains at least two distinct populations of antibodies with specificities for determinants on the group B capsular polysaccharide antigen. These two populations were differentiated on the basis of the ability of only one of them to be absorbed from the antiserum by the structurally related colominic acid. The nature of the colominic acid-specific determinant was elucidated by a radioimmunoassay inhibition technique with the use of a series of linear alpha-(2----8)-linked oligomers of sialic acid as inhibitors. Colominic acid was labeled by prior removal of its N-acetyl groups, followed by their replacement with the use of [3H]acetic anhydride. The conformational nature of the determinant was proposed because of the unusually large size (10 sialic acid residues) of the oligomer required to function as an efficient inhibitor. The structure of the determinant responsible for the second population of group B-specific antibodies has not been determined, but it is obviously based on an as yet undefined conformational or structural feature peculiar to the group B meningococcal polysaccharide. In contrast to the colominic acid-specific group B determinant, the determinant responsible for the group C polysaccharide-specific rabbit antibodies proved to be more conventional. Inhibitory properties of the alpha-(2----9)-linked oligomers maximized with those containing four or five sialic acid residues, which is consistent with the approximate estimated maximal size of an antibody site.