[Establishment of Helicobacter pylori infection model in Mongolian gerbil].
[Establishment of Helicobacter pylori infection model in Mongolian gerbil].
复制标题
沙鼠幽门螺杆菌感染模型的建立[J].
DOI：
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发表时间：
2003
影响因子：
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通讯作者：
Qiang Li
中科院分区：
文献类型：
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作者：
Jie Yan;Ai;Qiang Li
目的
目的建立稳定可靠的幽门螺杆菌感染蒙古沙土鼠模型，并观察胃粘膜的病理变化。
方法
将长爪沙鼠随机分为6组（N组，n=6）、6组（Y组，n=6）和6组（C组，n=4）。用含有片状血的哥伦比亚琼脂中的布鲁氏菌肉汤制备浓度为2 × 10（8）CFU/ml和2 × 10（9）CFU/ml的菌株NCTC 11637和菌株Y 06的幽门螺杆菌悬液。用0.5 ml 2 × 10（8）CFU/ml幽门螺杆菌悬液经口激发N组和Y组动物一次。另一组N和另一组Y动物分别用0.5 ml 2 × 10（9）CFU/ml幽门螺杆菌悬液经口攻击，每次间隔24 h，共3次。分别于末次感染后第2、4、6周处死动物，取胃粘膜标本进行尿素酶试验、细菌分离、常规病理和幽门螺杆菌组织化学检查。
结果
攻毒后第2、4、6周N组和Y组动物的感染率分别为0%、0%、66.7%和0%、16.7%、16.7%。N组和Y组在攻毒后第2、4、6周的感染率分别为66.7%、100%、100%和66.7%、66.7%、100%。细菌分离阳性动物胃粘膜细胞表面和胃小凹内有幽门螺杆菌定植，胃粘膜固有层有慢性炎症细胞浸润。
结论
用高浓度的1 × 10（9）CFU的H.pylori悬液多次经口攻击蒙古沙土鼠，可制备稳定可靠的H.pylori感染模型。幽门螺杆菌可在感染动物的胃粘膜中定植，并可见轻度炎症反应。
OBJECTIVE
To establish a stable and reliable model of Helicobacter pylori infection in Mongolian gerbil and to observe pathological changes in gastric mucosa from the infected animals.
METHODS
Mongolian gerbils were randomly divided into six groups infected with H.pylori strain NCTC11637 (n=6, group N), six groups infected with H.pylori clinical strain Y06 (n=6, group Y) and six groups as negative control (n=4, group C). H.pylori suspensions at the concentrations of 2 X 10(8)CFU/ml and 2 X 10(9) CFU/ml of strain NCTC11637 and strain Y06 were prepared with Brucella broth from Columbia agar containing sheet blood. The animals in one group N and in one group Y were orally challenged once with 0.5 ml of 2 X 10(8) CFU/ml H.pylori suspension. The animals in another group N and in another group Y were orally challenged with 0.5 ml of 2 X 10(9) CFU/ml H.pylori suspension for three times at the intervals of 24 hours, respectively. The animals were killed after 2nd, 4th and 6th week of the last infection and the gastric mucosal samples were taken for urease test, bacterial isolation, routine pathological and H.pylori histochemical examinations.
RESULTS
Infection rates of the animals in group N and group Y at the 2nd, 4th and 6th week after one challenge were 0%, 0%, 66.7% and 0%, 16.7%, 16.7%, respectively. Infection rates of the animals in groups N and Y at the 2nd, 4th and 6th week after three challenges were 66.7%, 100%, 100% and 66.7%, 66.7%, 100%, respectively. In animals with positive bacterial isolation H.pylori was found to colonized on the surface of gastric mucosal cells and in the gastric pits, and the lamina propria of gastric mucosal was infiltrated with chronic inflammatory cells.
CONCLUSION
By using H.pylori suspension at high concentration of 1 X 10(9) CFU for multiple times, the orally challenged Mongolian gerbils can be prepared as a stable and reliable H.pylori infection model. H.pylori can colonize in gastric mucosa of the infected animals, and mild inflammation reactions can be seen.