An evaluation of the interference of hydroxycobalamin with chemistry and co-oximetry tests on nine commonly used instruments
An evaluation of the interference of hydroxycobalamin with chemistry and co-oximetry tests on nine commonly used instruments
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DOI：
10.3109/00365513.2011.573573
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发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Szecsi, Pal B.
中科院分区：
文献类型：
--
作者：
Carlsson, Christian J.;Hansen, Heidi E.;Szecsi, Pal B.
单独或与硫代硫酸钠一起使用羟钴胺(OHCob)是氰化物中毒的标准治疗方法。OHCob是一种红色发色团，在一些相互矛盾的报告中，它对CO血氧和比色实验室测量的干扰进行了评估。用10种不同浓度的OHCob(0~1500 mg/L)对样品进行了OHCob干扰研究。使用9种不同的分析仪(ABL 800 Flex、Advia 1800、Advia Centaur XP、Architect ci8200、IMMULITE 2500、Konelab 30i、Modular Analytics SWA、Synchron LX 20和Vitros 5.1)测量73种不同分析物的浓度。所有仪器都产生了一些受OHCob影响的结果，其浓度相当于单一治疗剂量。在73种不同的分析物中，%至少对一种仪器有干扰。在所有187项测试中，47%是有偏见的，超过10%。干扰一般仅限于光度分析，而免疫学和离子选择电极测量不受影响。氰化物中毒治疗后出现在血液中的OHCob以一种不可预测的方式干扰了许多实验室分析，使一些结果无效。一些受影响的检测在氰化物中毒的治疗中很重要。这种干扰不仅是波长造成的，也是化学作用造成的。在不拖延OHCob给药的情况下，最好提前抽血，或者至少应该通知实验室有关OHCob治疗的情况。如果实验室收到含有OHCob的样品，应选择方法和仪器将偏差降至最低，OHCob制造商应在包装插页中向客户建议相关预防措施。
The administration of hydroxocobalamin (OHCob), alone or with sodium thiosulfate, is a standard therapy for cyanide poisoning. OHCob is a red chromophore, and its interference with co-oximetric and colorimetric laboratory measurements has been evaluated in a few conflicting reports. The interference of OHCob was investigated in samples spiked with 10 different concentrations of OHCob (0-1500 mg/L). The concentration of 73 different analytes was measured using nine different analysers (ABL 800 Flex, Advia 1800, Advia Centaur Xp, Architect ci8200, Immulite 2500, Konelab 30i, Modular Analytics SWA, Synchron LX 20 and Vitros 5.1). All instruments yielded some results that were affected by OHCob at concentrations equivalent to a single therapeutic dose. Of the 73 different analytes, 64% showed interference on at least one instrument. Of all 187 tests performed, 47% were biased with more than 10%. Interference was generally limited to photometric assays, whereas immunological and ion-selective electrode measurements were unaffected. OHCob present in the blood after treatment for cyanide poisoning interfered with many laboratory assays in an unpredictable way, making some results invalid. Some affected tests are important in the treatment of cyanide poisoning. The interference is not solely due to wavelength, but also to chemical interaction. Without delaying the administration of OHCob, blood should, preferably, be drawn in advance, or, at least, the laboratory should be informed about the OHCob treatment. If the laboratory receives OHCob-containing samples, methods and instruments should be selected to minimize bias, and the manufacturer of the OHCob should recommend relevant precautions to customers in the package insert.